Progress of Using Nanoparticles as Gene Vector

Abstract

Abstract: Nanoparticles have some characteristics such as can combine with big fragment DNA，low immunogenic，good biocompatibility，easy to produce，which can protect DNA against the digestion of nuclease. The nanoparticles have a high stability in organisms and cells，which was difficult to be degraded. Nanoparticles can improve transfection efficiency. As a nonviral gene vector，the application of nanoparticles in gene therapy became research focus. In this article，we mainly discussed the characteristics of nanoparticles， characteristics and transport mechanism of nanoparticles as gene vector，the progress on nanoparticles as gene vector.

Key words: Nanoparticles, Gene vectors, Transfection

Lu Yanmin. Progress of Using Nanoparticles as Gene Vector[J]. Biotechnology Bulletin, 2013, 0(2): 61-66.

References

［1] Fishlock TW, Oral A, Egdell RG, et al. Manipulation of atoms across a surface at room temperature［J]. Nature, 2000, 404(6779)： 743-745.
[2] Pascal A, Franklin AH, Eric W, et al. Halogen bonds in biological molecules［J]. Proc Natl Acad Sci USA, 2004, 101(48)：16789- 16794.
[3] 张阳德. 纳米生物技术学［M]. 北京：科学出版社, 2005:62.
[4] Chilkoti A, Dreher MR, Meyer DE. Design of thermally responsive, recombinant polypeptide carriers for targeted drug delivery［J]. Adv Drug Deliv Rev, 2002, 54(8)：1093-1111.
[5] Maillard S, Ameller T, Gauduchon J, et al. Innovative drug delivery nanosystems improve the anti-tumor activity in vitro and in vivo of anti-estrogens in human breast cancer and multiple myeloma［J]. J Steroid Biochem Mol Biol, 2005, 94(1-3)：111-121.
[6] Yin WK, Feng SS. Effects of particle size and surface coating on cellular uptake of polymeric nanoparticles for oral delivery of anticancer drugs［J]. Biomaterials, 2005, 26(15)：2713-2722.
[7] Mari T, Masanori U, Noritada K, et al. Nanospheres for DNA separation chips［J]. Nat Biotech, 2004, 22(3)：337-340.
[8] Gao XH, Cui YY, Levenson RM, et al. In vivo cancer targeting and imaging with semiconductor quantum dots［J]. Nat Biotech, 2004, 22(8)：969-976.
[9] James JS, Adam DL, Viswanadham G, et al. Homogeneous detection of unamplified genomic DNA sequences based on colorimetric scatter of gold nanoparticle probes［J]. Nat Biotech, 2002, 22(7)： 883-887.
[10] Medintz IL, Konnert JH, Clapp AR, et al. A fluorescence resonance energy transfer-derived structure of a quantum dot-protein bioconjugate nanoassembly［J]. Proc Natl Acad Sci USA, 2004, 101(26)：9612-9617.
[11] Zhao XJ, Hilliard LR, Mechery SJ, et al. From the cover:A rapid bioassay for single bacterial cell quantitation using bioconjugated nanoparticles［J].Proc Natl Acad Sci USA, 2004, 101(42)： 15027-15032.
[12] 肖苏尧, 刘选明, 童春义, 等. 多聚赖氨酸淀粉纳米颗粒基因载体的研制及应用［J]. 中国科学B 辑, 2004, 34(6)：473- 477.
[13] Indrajit R, Tymish YO, Dhruba JB, et al. Optical tracking of organically modified silica nanoparticles as DNA carriers:A nonviral, nanomedicine approach for gene delivery［J]. Proc Natl Acad Sci USA, 2005, 102(2)：279-284.
[14] Klabunde KJ, Stark J, Koper O, et al. Nanocrystals as stoichiometric reagents with unique surface chemistry［J]. J Phys Chem, 1996, 100(30)：12142-12153.
[15] Golabi SM, Nozad A. Electrocatalytic oxidation of methanol on electrodes modified by platinum microparticles dispersed into poly (o-phenylenediamine)film［J]. J Joumal of Electroanalytical Chemistry, 2002, 521(1-2)：161-167.
[16] Alivisatos AP. Semiconductor clusters, nanocrystals, and quantum dots［J]. Science, 1996, 271:933-937.
[17] Jiang P, Liu ZF, Cai SM. In situ CdS nanocluster formation on scanning tunneling microscopy tips for reliable single-electron tunneling at room temperature［J]. Applied Physics Letters, 1999, 75(19)：3023-3025.
[18] Luo D, Saltzman WM. Synethetic DNA delivery systems［J].Nat Biotech, 2000, 18:33-37.
[19] Lehrman S. Virus treatment questioned after gene therapy death[J]. Nature, 1999, 401(6753)：517- 518.
[20] Van Craynest N, Santaella C, Boussif O, Vierling P. Polycationic telomers and cotelomers for gene transfer:synthesis and evaluation of their an vitro transfection efficiency［J]. Bioconjug Chem, 2002, 13(1)：59-75.
[21] Junghans M, Kreuter J, Zimmer A. Antisense delivery using protamine-oligonucleotide particles［J]. Nucleic Acids Res, 2000, 28(10)：e45.
[22] Jeong JH, Park TG. Poly(L-lysine)-g-poly(D, L-lactic-co-glycolic acid)micelles for low cytotoxic biodegradable gene delivery carriers［J]. J Control Release, 2002, 82(1)：159-166.
[23] Ogirs M, Wagner E. Targeting tumors with non-viral gene delivery systems［J]. Drug Discovery Today, 2002, 7(8)：479-485.
[24] Chan CK, Senden T, Jans DA. Supramolecular structure and nuclear targeting efficieney determine the enhancement of transfection by 2013年第2期65 卢艳敏：纳米基因载体研究进展 modified polylysines［J]. Gene Therapy, 2000, 7:1690-1697.
[25] Kneuer C, Sameti M, Haltner EG, et al. Silica nanoParticles modified with aminosilanes as carriers for plasmid DNA［J]. International Journal of Pharmaceutics, 2000, 196(2)：257-261.
[26] Fenske DB, Maclachlan I, Cullis PR, et al. Long-circulating vecots for the systemic delivery of genes［J].Curr Opin Mol Ther, 2000, 3(2)：153-158.
[27] Schatzlein AG. Targeting of synthetic gene delivery systems［J]. J Biomed Biotechnol, 2003(2)：149-158.
[28] Jackson DA, Juranek S, Lipps HJ. Designing nonviral vectors for efficient gene transfer and long-term gene expression［J].Mol Ther, 2006, 14(5)：613-626.
[29] Pack DW, Hoffman AS, Pun S, et al. Design and development of polymers for gene delivery［J]. Nat Rev Drug Discov, 2005, 4(7)： 581-593.
[30] Truong-Le VL, August JT, Leong KW. Controlled gene delivery by DNA-gelatin nanospheres［J]. Hum GeneTher, 1998, 9:1709- 1717.
[31] Ma H, Diamond SL. Nonviral gene therapy and its deliverysystems[J]. Curr Pharm Biotechn, 2001, 2(1)：1-17.
[32] Moghimi SM, Hunter AC, Murray JC. Long-circulating and targetspecific nanoparticles:theory to practices［J]. Pharmacol Rev, 2001, 53(3)：283-318.
[33] Ciftci K, Levy RJ. Enhanced plasmid DNA transfection with lysosomotropic agents in cultured fibroblasts［J]. Inter J Pharm, 2001, 218(1-2)：81-92.
[34] Lambert G, Fattal E, Couvreur P. Nanoparticulate systems for the delivery of antisense oligonucleotides［J]. Advanced Drug Delivery Reviews, 2001, 47(1)99-112.
[35] Braun CS, Vetro JA, Tomalia DA, et al. Structure/function relationships of polyamidoamine/DNA dendrimers as gene delivery vehicles［J]. J Pharm Sci, 2005, 94(2)：423-436.
[36] El-Sayed M, Ginski M, Rhodes C, et al. Transepithelial transport of poly(amidoamine)dendrimers across Caco-2 cell monolayers［J]. J Control Release, 2002, 81(3)：355-365.
[37] Kihara F, Arima H, Tsutsumi T, et al. Effects of structure of polyamidoamine dendrimer on gene transfer efficiency of the dendrimer conjugate with alpha-cyclodextrin［J]. Bioconjug Chem, 2002, 13(6)：1211-1219.
[38] Luo D, Haverstick K, Belcheva N, et al. Poly(ethylene glycol)- conjugated PAMAM dendrimer for biocompatible, high-efficiency DNA delivery［J]. Macromolecules, 2002, 35(9)：3456-3462.
[39] Liu Y, Jia S, Wu Q, et al. Studies of Fe3O4-chitosan nanoparticles prepared by co-precipitation under the magnetic field for lipase immobilization［J]. Catalysis Communications, 2011, 12(8)： 717-720.
[40] Waite C, Sparks S, Uhrich K, et al. Acetylation of PAMAM dendrimers for cellular delivery of siRNA［J].BMC Biotechnology, 2009, 9:38.
[41] Patil ML, Zhang M, Taratula O, et al. Internally cationic polyamidoamine PAMAM-OH dendrimers for siRNA delivery:effect of the degree of quaternization and cancer targeting［J]. Biomacromolecules, 2009, 10(2)：258-266.
[42] Zhang X, Oulad-Abdelghani M, Zelkin AN, et al. Poly(l-lysine) nanostructured particles for gene delivery and hormone stimulation[J]. Biomaterials, 2010, 31(7)：1699-1706.
[43] Cho KC, Kim SH, Jeong JH, et al. Folate receptor-mediated gene delivery using folate-poly(ethylene glycol)-poly(L-lysine) conjugate［J]. Macromol Biosci, 2005, 5(6)：512-519.
[44] Deng R, Yue Y, Jin F, et al. Revisit the complexation of PEI and DNA-How to make low cytotoxic and highly efficient PEI gene transfection non-viral vectors with a controllable chain length and structure?[J]. Journal of Controlled Release, 2009, 140(1)：40-46.
[45] Tagawa T, Manvell M, Brown N, et al. Characterisation of LMD virus-like nanoparticles self-assembled from cationic liposomes, adenovirus core peptide mu and plasmid DNA［J]. Gene Ther, 2002, 9(9)：564-576.
[46] Verkman AS, Sonawane ND, Szoka FC. Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes［J]. J Biol Chem, 2003, 278(45)：44826-44831.
[47] Elfinger M, Geiger J, Hasenpusch G, et al. Targeting of the β2-adrenoceptor increases nonviral gene delivery to pulmonary epithelial cells in vitro and lungs in vivo［J]. Journal of Controlled Release, 2009, 135:234-241.
[48] Jeong JH, Lee M, Kim WJ, et al. Anti-GAD antibody targeted nonviral gene delivery to islet beta cells［J]. J Control Release, 2005, 107(3)：562-570.
[49] Kunath K, Merdan T, Hegener O, et al. Integrin targeting using RGD-PEI conjugates for in vitro gene transfer［J]. J Gene Med, 2003, 5(7)：588-599. 生物技术通报 Biotechnology Bulletin 2013年第2期66
[50] Elfinger M, Maucksch C, Rudolph C. Characterization of lactoferrin as a targeting ligand for nonviral gene delivery to airway epithelial cells［J]. Biomaterials, 2007, 28(23)：3448-3455.
[51] Weiss SI, Sieverling N, Niclasen M, et al. Uronic acids functionalized polyethy- leneimine(PEI)-polyethyleneglycol(PEG)-graftcopolymers as novel synthetic gene carriers［J]. Biomaterials, 2006, 27(10)：2302-2312.
[52] Chul Cho K, Hoon Jeong J, Jung Chung H, et al. Folate receptormediated intracellular delivery of recombinant caspase-3 for inducing apoptosis［J]. J Control Release, 2005, 108(1)：121-131.
[53] Liu WG, Yao KD. Chitosan and its derivatives-a promising nonviral vector forgene transfection［J]. J Control Release, 2002, 83 (1)：1-11.
[54] Mansouri S, Lavigne P, Corsi K, et al. Chitosan-DNA nanoparticles as non-viral vectors in gene therapy:strategies to improve transfection efficacy［J]. Eur J Pharm Biopharm, 2004, 57(1)：1-8.
[55] Qin F, Zhou Y, Shi J, et al. A DNA transporter based on mesoporous sili-ca nanospheres mediated with polycation poly(allylaminehydrochloride) coating on mesopore surface［J]. Biomed Mater Res, 2009, 90A:333.
[56] Mahmoudi M, Simchi A, Imani M, et al. Superparamagnetic iron oxide nanoparticles with rigid cross-linked polyethylene glycol fumarate coating for application in imaging and drug delivery［J]. J Phys Chem, 2009, 113(19)：8124-8131.
[57] Qureshi HY, Ahmad R, Zafarullah M. High-efficiency transfection of nucleic acids by the modified calcium phosphate precipitation method in chondrocytes［J]. Anal Biochem, 2008, 382(2)： 138-140.
[58] Sandhu KK, McIntosh CM, Simard JM, et al. Gold nanoparticles mediated transfection of mammalian cells［J]. Bioconjugate Chem, 2002, 13(1)：3-6.
[59] Saccardo P, Villaverde A, González-Montalbán N. Peptide-mediated DNA condensation for non-viral gene therapy［J]. Biotechnology Advances, 2009, 27(4)：432-438. (责任编辑狄艳红)

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