Fusion Expression of Ferritin and Foot-and-Mouth Disease Virus VP1 in Escherichia coli and Self-assembly of Nanoparticles

doi:10.13560/j.cnki.biotech.bull.1985.2020-0758

Abstract

Abstract:

Foot-and-mouth disease is one of the most important livestock diseases in the world caused by foot-and-mouth disease virus,which seriously affects the development of animal husbandry in the world,and vaccine immunization is still the most effective means for the prevention and control of the epidemic. Ferritin has the characteristics of self-assembly and biological modification,and has broad application prospects in the field of nano vaccines. In this study,the vp1 gene of type O foot-and-mouth disease virus and the Helicobacter pylori ferritin gene were selected for nanoparticles assembly. The vp1 gene was fused to ferritin subunit gene by overlap PCR. After being expressed in Escherichia coli,it was purified by nickel column affinity chromatography by His Tag. The purified recombinant protein was then subjected to Western blotting detection,mass spectrometry analysis and transmission electron microscopy observations. It was found that the recombinant protein VP1-Ferritin was expressed in E. coli and self-assembled into nanoparticles.

Key words: foot-and-mouth disease virus, ferritin, nanoparticles, self-assemble

ZHANG Wei-ye, SONG Hao-zhi, LIU Xing-jian, LI Yi-nü, ZHANG Zhi-fang. Fusion Expression of Ferritin and Foot-and-Mouth Disease Virus VP1 in Escherichia coli and Self-assembly of Nanoparticles[J]. Biotechnology Bulletin, 2021, 37(2): 96-102.

Figures/Tables 9

References 32

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引物名称	引物序列（5'-3'）
F1	CGGGATCCAACATGACCACCTCCACAGGTGAGTC
R1	GCGTCGACGAATTCTTAGCTCTTGCGGGACTTGGCGATG
F2	CCTGTGAAACAGTTGTTG TCCGGTGGCGACATCATCAAG
R2	CTTGATGATGTCGCCACCGGACAACAACTGTTTCACAGG

引物名称	引物序列（5'-3'）
F1	CGGGATCCAACATGACCACCTCCACAGGTGAGTC
R1	GCGTCGACGAATTCTTAGCTCTTGCGGGACTTGGCGATG
F2	CCTGTGAAACAGTTGTTG TCCGGTGGCGACATCATCAAG
R2	CTTGATGATGTCGCCACCGGACAACAACTGTTTCACAGG