Protein Fragment Complementation Assay（PCA）and the Applications

Abstract

Abstract: Protein fragment complementation analysis (PCAs) is a recently developed new technique to assay protein-protein interactions. Typical PCA has been using dihydrofolate reductase, β-lactamase, GFP and luciferases to study protein-protein interactions successfully. This technology can not only analysis the localization of protein molecular interaction dynamically, draw intracellular signal transduction, protein biological chemical network, but also can be applied to protein library and high throughput new drug screening. In this review, we summarized the concept, methodology and the applications of PCA.

Key words: Protein-protein interaction, Protein fragment complementation assay, High-throughput screening in drugg

Zhang Jie, Liu Changbai. Protein Fragment Complementation Assay（PCA）and the Applications[J]. Biotechnology Bulletin, 2013, 0(2): 67-71.

References

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