Isolation,Culture and Identification of Bovine Mammary Epithelial Cell

Abstract

Abstract: It was to set up a simple, economic and pure method to culture and identify bovine mammary epithelial cell（BMEC）in vitro, which were collected from centrifuging the fresh milk. Then, the cell growth curve, keratin8, keratin18, Beta casein and karyotype were identified. After three days, several cells grew together, and the shape looked like typical paving stones. At the same time, there were also a few phagocytes. Continue to culture several days, all cells grew together, and there were some emulsion droplets secreted to the outside of the cells with almost all phagocytes transferring into the emulsion droplets. When the cells were propagated, all phagocytes were disappeared. The cell growth curve was “S”. Keratin8, Keratin18 and Beta casein had been detected through the method of RT-PCR. Keratin8 were also detected by immunohistochemistry. The karyotype result was the same as bovine’s.

Key words: Freash milk, Bovine mammary epithelial cell, Cytoskeletal protein, Lactation

Cui Xinjie, Wang Ting, Liu Bingchun, Tao Lin, Wang Xiao. Isolation,Culture and Identification of Bovine Mammary Epithelial Cell[J]. Biotechnology Bulletin, 2013, 0(3): 107-113.

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