Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (3): 181-185.

• Research report • Previous Articles     Next Articles

Screening and Application of the Antagonistic Bacterium HY32 Against Colletotrichum gloeosporioides

Lu Zongyan, Zhou Guoying, Chen Yuhua, Yan Faling, Yan Ruikun, Wu Nan   

  1. College of Forestry,Central South University of Forestry and Technology,Changsha 410004)
  • Received:2012-12-06 Revised:2013-03-21 Online:2013-03-20 Published:2013-03-21

Abstract: We isolated pathogenic bacteria by means of tissue isolation. Through morphology, pathogenicity determination and phylogenetic analysis, it showed that the cause of fir anthrax pathogen is Colletotrichum gloeosporioides. Acquisition of Sugiki Kenkosugimie, through the flat confrontation method screening, fermentation liquid compound sieve, we got 1 strains with strong antagonistic activity of bacteria, numbered HY32, bacteriostasis diameter achieving above of 10 mm, fermentation broth bacteriostasis activity up to 81.7%. Culture tests showed it had strong antagonistic activity to 4 plant pathogens(Cunninghamia lanceolata, Agaricodochium camellia, Fusarium proliferatum, Pestalotio psismicrospor). Through indoor potted test of the HY32, the results showed that compared with the control group the HY32 has significant control effect against Chinese fir anthracnose, reaching 60.0%.

Key words: Chinese fir anthracnose, Pathogen, Antagonistic bacteria, Screening