Pilot Preparation and Optical Properties of Highly Purified Phycoerythrin from Porphyra yezoensis

Abstract

Abstract: Pilot preparation were tried to obtain highly purified phycoerythrin（PE）in abundance from P. yezoensis in Lvsi city, Jiangsu Province and to study its optical properties. 2 kg freeze-dried P. yezoensis thallus was broken using “swelling & smash” method. Further purification was performed by means of ammonium sulphate co-precipitation（ASCP）and hydroxyapatite（HA）column chromatogram and the purity of PE achieved 1.67（A565/A280）, corresponding to yield of 0.184%, The purity of PE could be improved beyond 4.5 with a yield of 0.109% if HAP chromatography repeated. PE was validated by absorption/fluorescence spectra and SDS-PAGE/Native-PAGE stained with coomassie brilliant blue/zinc acetate for its purity. Molar extinction coefficient（MEC）of PE were 1.79mL/（mg?cm）and 1.73 mL/（mg?cm） determined by folin-phenol method and Bradford method, respectively. Fluorescence quantum yield of PE was 0.90 at 505 nm wavelength at 20℃ . The PE effect of photo bleaching was the most significant under irradiating of laser among daylight lamp, light emitting diode（LED）, iodine-tungsten lamp and laser. For example, the photo bleaching rate of 240 μg/mL PE after 10 minutes’ irradiating under laser was close to the value using iodine-tungsten lamp of 240 minutes’ irradiating.

Key words: Porphyra yezoensis, Phycoerythrin, Purification, Molar extinction coefficient, Fluorescence quantum yield,Photobleaching

Guo Ziye, Cai Chuner, Li Chunxia, Geng Zhonglei, Jia Rui, He Peimin, . Pilot Preparation and Optical Properties of Highly Purified Phycoerythrin from Porphyra yezoensis[J]. Biotechnology Bulletin, 2013, 0(3): 192-198.

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