Genetic Diversity and Clustering Analysis on 48 Olive Cultivars

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (3): 96-101.

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Genetic Diversity and Clustering Analysis on 48 Olive Cultivars

Chen Haiyun¹, Chen Shaoyu¹, Ning Delu², Li Rui¹, Li Yongjie², Mao Yunling¹, Wu Tao1

1. Key Laboratory of Forest Plant Cultivation and Utilization,Yunnan Academy of Forestry,Kunming 650204 ；2. Institute of Economic Forest Trees,Yunnan Academy of Forestry,Kunming 650204）

Received:2012-09-24 Revised:2013-03-21 Online:2013-03-20 Published:2013-03-21

Abstract

Abstract: Inter-simple sequence repeat（ISSR）molecular markers were applied to analyze genetic diversity and clustering of 48 introduced and breeding olive cultivars. 106 bands were amplified by 11 selected primers,among which 99 bands were polymorphic. Percentage of polymorphic loci was 93.40%,which showed a rich genetic diversity in the collection. Based on Nei’s genetic distances,dendrogram of 48 cultivars was constructed through unweighted pair-group method（UPMGA）. 48 cultivars were clustered into 4 main groups. 84.6% of native select and breeding cultivars were clustered into 2 groups and most of introduced cultivars were clustered based on their directly introducing places and main usage. The results will provide references for olive resource utilization and further genetic improving.

Key words: Olea euyopaea, L. Genetic diversity, Clustering analysis

Chen Haiyun, Chen Shaoyu, Ning Delu, Li Rui, Li Yongjie, Mao Yunling, Wu Tao. Genetic Diversity and Clustering Analysis on 48 Olive Cultivars[J]. Biotechnology Bulletin, 2013, 0(3): 96-101.

References

[1] 吴开志, 肖千文, 贾瑞芬, 等. 油橄榄品种表型性状的多样性[J]. 经济林研究, 2008, 26(2):48-52.
[2] 姜成英, 戚登臣, 苏瑾. 甘肃省油橄榄生产现状与发展对策[J]. 经济林研究, 2006, 24(2):78-81.
[3] Belaj A, Satovic Z, Ralh L, et al. Optimal use of RAPD markers for identifying varieties in olive(Olea europaea L.)germplasm collections [J]. J Amer Soc Hort Sci, 2004, 129(2):266-270.
[4] Hagidinitriou M, Katsiotis A, Menexes G, et al. Genetic diversity of major Greek Olive cultivars using molecular(AFLP and RAPDs) markers and morphorlogical traits[J]. J Amer Soc Hort Sci, 2005, 130(2):211-217.
[5] Owen CA, Bita EC. Banilas G, et al. AFLP revealed structural detail of genetic diversity within cultivated olive germplasm from the Eastern Mediterranean[J]. Theor Appl Genet, 2005, 110 :1169-1176.
[6] Montemurro C, Sineone R, Pasqualone A, et al. Genetic relationships and cultivar identification among112 olive accessions using AFLP and SSR markers[J]. Hort Sciences, 2005, 80(1):105-110.
[7] Bemard R, Manzo M, Durante M, et al. Molecular markers for cultivar characterization in Olea europaea[J]. Acta Horticulturae, 2002, 586 :97-100.
[8] 邱源, 韩华柏, 李俊强. 23 个油橄榄品种的RAPD 分析[J]. 林业科学, 2008, 44(1):85-59.
[9] 马万里, Collins G. 运用RAPD 技术鉴定澳大利亚油橄榄品种的研究[J]. 内蒙古师范大学学报:自然科学汉文版, 2006, 35(3): 337-339.
[10] 李瑞, 陈少瑜, 宁德鲁, 等. 油橄榄ISSR-PCR 反应体系优化与引物筛选[J]. 安徽农业科学, 2012(10):5797-5799.
[11] Martins-Lopes P, Lima-Brito J, Gomes S, et al. RAPD and ISSR molecular markers in Olea europaea L. :Genetic variability and molecular cultivar identification[J]. Genetic Resources and Crop Evolution, 2007, 54 :117-128.
[12] Claros GM, Crespillo R, Aguilar ML. et al. DNA fingerprinting and classification of geographically related genotypes of olive tree(Olea europaea L.)[J]. Euphytica, 2000, 116 :131-142.
[13] Besnard G, Breton C, Baradat P, et al. Cultivar identification in olive based on RAPD markers[J]. J Amer Soc Hort Sci, 2002, 126 : 668-675.
[14] Rotondi A, Magli M, Ricciolini C, et al. Morphological and molecular analyses for the characterization if a group of Italy olive cultivars [J]. Euphytica, 2003, 132 :129-137.

Genetic Diversity and Clustering Analysis on 48 Olive Cultivars

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