Optimizing Method of Preparation of Protoplast in Fusedum oxysporum f. sp. capsicum

Abstract

Abstract: Several conditions in preparing the protoplast of Fusedum oxysporum f. sp. capsicum were optimized. Effects of the incubation time, enzyme system, the enzymolysis time, temperature and rotate speed were discussed. It was found that when keeping other parameters as : spore cultured for 14 hours, 15 mg/mL of Drislase, 0.7 mol/L NaCl and 140 r/min, 32℃ for 4 h, the minimum consummation of protoplasts can be obtained and the regeneration rate of the protoplast was 32.3% on regeneration medium SR.

Key words: Fusedum oxysporum f. sp. capsicum, Preparation of protoplast, Regeneration

Mi Baobin, Zhang Jixiang, Yang Yuhong, Mao Zhenchuan. Optimizing Method of Preparation of Protoplast in Fusedum oxysporum f. sp. capsicum[J]. Biotechnology Bulletin, 2013, 0(4): 96-100.

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