Construction and Preliminary Analysis of cDNA Library of Puccinellia tenuiflora Roots Grown in Saline-alkaline Land

Abstract

Abstract: Puccinellia tenuiflora roots gathered from saline-alkaline land were used to construct a cDNA library by using SMAT technology. The titer of the amplified cDNA library was 1.747×10⁹ CFU/mL, the inserted fragments were distributed from 0.5 kb to 2 kb, and the recombinant rate is 92%. Preliminary analysis of ESTs indicated that S-adenosylmethionine synthetase 2 gene and caltractin gene associated closely with plant salt-responsive reactions were screened in this cDNA library, which can be used to further screen salt-tolerant genes from Puccinellia tenuiflora. This cDNA library of Puccinellia tenuiflora roots laid important foundations for revealing salt tolerant mechanism in Puccinellia tenuiflora, mining salt tolerant genes, and cultivating engineered salt-tolerant plants.

Key words: Saline-alkaline land, Puccinellia tenuiflora, Roots, cDNA library, ESTs

Fu Chang, Wang Shengnan, Guo Min. Construction and Preliminary Analysis of cDNA Library of Puccinellia tenuiflora Roots Grown in Saline-alkaline Land[J]. Biotechnology Bulletin, 2013, 0(5): 69-72.

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