Research Progress in Chitinase Involving in the Biocontrol of Crop Diseases and Pests

doi:10.13560/j.cnki.biotech.bull.1985.2023-0520

Abstract

Abstract:

Pests and diseases seriously affect the quality, yield and safety of crops. As a green, safe and effective method to control pests and diseases, biological control has attracted more and more attention. Chitinase（EC 3.2.1.14）is a kind of glycosylated hydrolase widely existing in microorganisms and plants. It can effectively degrade the chitin in cell walls of pathogenic fungi, insects and nematodes causing diseases and insect pests, and inhibit the spore germination and mycelium growth of pathogenic fungi, and the development of insects and nematodes, which has an important role and application prospect in the biological control of crop diseases and insect pests. This paper reviewed the types and sources of chitinases, the biological control mechanism and control strategy of chitinase, and the application of transgenic technology based on chitinase in crop pest control，aiming to provide information for further investigation and application of chitinase in the fields of crop production, pests and diseases control.

Key words: crops, plant diseases and insect pests, biological control

MA Sai-mai, LI Tong-yuan, MA Yan-jun, HAN Fu-jun, PENG Hai, KONG Wei-bao. Research Progress in Chitinase Involving in the Biocontrol of Crop Diseases and Pests[J]. Biotechnology Bulletin, 2023, 39(10): 29-40.

Figures/Tables 4

Fig. 1 Structural overview of the family 18 chitinases of S. marcescens The picture shows the crystal structures of ChiA, ChiB and the catalytic domain of ChiC, and the NMR structures of homologues of the FnⅢ and the CBM5/12 domain in ChiC. Helices are colored dark blue, β-strands are colored cyan. The side chain of the catalytic acid（a Glu）is shown in yellow

Fig. 2 Three-dimensional model of NbchiA A: Cartoon representation of the overall fold of NbchiA model（orange）and superimposition of NbchiA and S. coelicolor chitinase（purple）monomers. B: Molecular surface of the NbchiA model, all of the residues related to catalysis and binding are located in a pocket

Fig. 3 General structure of the fungal cell wall

Fig. 4 Presence of chitin layers（cuticle and gut）in case of insects

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