Identification on a Bacterial Strain HB-1 Separated from Stratum Water in Huabei Oilfield

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (6): 110-115.

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Identification on a Bacterial Strain HB-1 Separated from Stratum Water in Huabei Oilfield

Li Qing¹ Liu Yang² Wu Gang¹ Du Huijing² You Jing¹ Li Hong² Ke Congyu¹ Zhang Xin² Yu Jiliang¹ Zhao Ting²

(1. Petroleum Production Engineering Research Institute of Huabei Oilfield Company, Renqiu 062552;2. China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing 100027)

Received:2013-06-20 Revised:2013-06-20 Online:2013-06-20 Published:2013-06-20

Abstract

Abstract: A bacterial strain HB-1 was isolated from stratum water in Huabei Oilfield, and identificated by polyphasic taxonomy basing on morphologic and physiologic methods, chemical composition analysis, genetic characteristics test and 16S rRNA gene phylogenetic analysis. The result showed that the strain was a member of the genus Chelatococcus, and was belong to the known species of Chelatococcus daeguensis. 37℃ was its optimum growth temperature. The major polar lipids presented in strain HB-1 were DPG, PE, PG and PC. Major fatty acids were C_{18: 0} ω7c(39.49%)and C_{19: 0} cyclo ω8c(30.39%), and ubiquinone Q-10 was the predominant quinone. HB-1 had enzyme activity of oxidase, catalase, esterase(C4), esterase lipase(C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, acid phosphatase and naphthol-AS-BI-phosphate hydrolase. Chelatococcus daeguensis HB-1 was isolated from stratum water in Huabei Oilfield for the first time.

Key words: Huabei Oilfield, Stratum water, Chelatococcus daeguensis, HB-1, Polyphasic taxonomy

Li Qing, Liu Yang, Wu Gang, Du Huijing, You Jing, Li Hong, Ke Congyu, Zhang Xin, Yu Jiliang, Zhao Ting. Identification on a Bacterial Strain HB-1 Separated from Stratum Water in Huabei Oilfield[J]. Biotechnology Bulletin, 2013, 0(6): 110-115.

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Identification on a Bacterial Strain HB-1 Separated from Stratum Water in Huabei Oilfield

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