Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (6): 147-154.

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Reliability Study on the Genus Bacillus Species Based on Fatty Acid Identification

Liu Guohong1, 2 Liu Bo1 Lin Naiquan2   

  1. (1. Institue of Agricultural-Resource Fujian Academy of Agricultural Sciences, Fuzhou 350003;2. Fujian Agriculture and Forest University, Fuzhou 350002)
  • Received:2013-06-20 Revised:2013-06-20 Online:2013-06-20 Published:2013-06-20

Abstract: In this study, 10 type Bacillus species were selected and carried out a different repeats of fatty acid respectively using Sherlock MIS system. From the identitication results, similarity index(SI), fatty acid content and clustering analysis, it was showed that all species were accurately identified as species level, SI had a good reproducibility, the fatty acid content was little changed in each pepetition, and was no effection on the taxonomic status of Bacillus species by clustering analysis. Finally, the results were determined that the Bacillus fatty acid identification had a good reliability.

Key words: Bacillus, Fatty acid, Reliability, Sherlock, MIS system