Study on Characteristics of Constitutive Expression of GbLTP1 and GbLTP3 Genes in Land-cotton

Abstract

Abstract: According to the conserved sequences of LTPs gene from G. barbadense variety Xinhai 21 clone two lipid transferase gene GbLTP1 and GbLTP3. Constructing the constitutive expression vector pCAMBIA2301-GbLTP1 and pCAMBIA2301-GbLTP1 two plant expression vectors were transformed into upland cotton. By kanamycin screening, PCR and RT-PCR technique, to obtain transgenic GbLTP1 cotton and transgenic GbLTP3 cotton in all two strains. The progeny of transgenic plants disease resistance, agronomic and economic traits analysis, results showed that: the expression of GbLTP3 and GbLTP1 gene and Xinjiang upland cotton resistance to Fusarium wilt, resistance to yellow, and no significant increase of cotton. Effects of exogenous GbLTP3 gene and GbLTP1 gene into not agronomic traits, yield traits of cotton fiber quality, especially on fiber length has a certain improving effect.

Key words: Lipid transfer protein, Transgenic cotton, Cotton fusarium wilt and verticillium wilt, Agronomic characters, Fiber quality

Shen Haitao, Wang Aiying, Li Yuxia, Zhu Jianbo. Study on Characteristics of Constitutive Expression of GbLTP1 and GbLTP3 Genes in Land-cotton[J]. Biotechnology Bulletin, 2013, 0(6): 87-93.

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