The Effect of Physiological Traits of Transgenic Cotton Induced by Verticillium wilt Pathogen

Abstract

Abstract: To provide theoretical basis for improving resistant in transgenic cotton by researching the effect of physiological traits of transgenic cotton induced by Verticillium wilt pathogen. Chitinase and β-1, 3-glucan of bivalent resistance genes were delivered to two cotton lines of Byd, 28p by pollen-tube pathway method. The transgenic cotton lines with different resistant levels were obtained by many years screening. After the Verticillium wilt pathogen infestation to cotton seeds, the physiological and biochemical changes in transgenic cotton were determined at seedling stage. The results showed that the enzyme activity in transgenic disease-resistant cotton plants had reached the control's level, however, the level of enzyme activity in susceptible and disease-resistan cotton plants were different. The enzyme activity and changes of proline content were related to disease resistance of transgenic cotton plants.

Key words: Verticillium wilt, Disease resistance, Enzyme activity, Physiological index

Ou Xiuling, Geng Yawen, Li Feng, Li Caolong, Zhu Jianbo, Wang Aiying. The Effect of Physiological Traits of Transgenic Cotton Induced by Verticillium wilt Pathogen[J]. Biotechnology Bulletin, 2013, 0(6): 94-98.

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