Abstract: In this study, we collected 12 Urticaceae plants from Lushan mountain area to carry out the study of internal transcribed spacer（ITS）sequences, and thus further explore the feasibility as a study of the Urticaceae Phylogeny using ITS sequences, as well as the similarities and differences of traditional plant taxonomy and molecular systematics. We obtained the sequences of nrDNA ITS from 10 species of Urticaceae by PCR amplification and clone sequencing. For phylogenetic analyses using MEGA 4.0 software, both NJ and MP trees of Urticaceae were constructed with U.marirei as outgroup. The two methods produced trees with absolutely congruent topology. These phylogenetic trees showed three separate lineages：（1）Elatostemeae, （2）Boehmerieae, （3）Urticeae. The results suggested that it is feasible to research the phylogenetic of Urticaceae plants using ITS sequences. The results were basically consistent about the phylogenetic using molecular techniques and traditional morphology classification.

Key words: nrDNA, ITS, Urticaceae, PCR amplication, Phylogenetic analysis