Study on the Related Enzyme Activities in the Residues of Different Mushroom Substrates Inoculated with Cellulomonas uda and Trichoderma viride

Abstract

Abstract: Enzyme production test and collapse of filter paper test were used to establish the cellulase activity and the collapse degree of filter paper with single strain of cellulolytic bacterium(Cellulomonas uda)and fungus(Trichoderma viride). Then mixing ratios of cellulolytic bacterium and fungus were determined to degrade the residues of different mushroom substrates in the liquid fermentation on the basis of the collapse degree of filter paper and carboxymethyl cellulase(CMCase)activity, to study on the optimal mixing ratio of cellulolytic bacterium and fungus and change of related enzyme activities in the fermentation of residues of mushroom substrates. The results showed that the CMCase activity of Cellulomonas uda was higher than those of Trichoderma viride in both the cellulase production medium and collapse of filter paper medium. However, the mixed fermentation of Cellulomonas uda and Trichoderma viride greatly improved the cellulase enzyme activity. After four days liquid fermentation, using eucalypt sawdust-pleurotus geesteranus, mixed sawdust-ganoderma and sawdust-pleurotus geesteranus as only carbon source, the maximum CMCase activity was 2 809, 3 606 and 971 U/mL and the maximum filter paper(FPase)activity was 486, 454 and 151 U/mL, respectively. These results can provide basis for the large scale solid state fermentation, and the suitable mixing ratio of Cellulomonas uda and Trichoderma viride for eucalypt sawdust-pleurotus geesteranus, mix sawdust-ganoderma and mixed sawdust-pleurotus geesteranus was 1∶ 3, 3∶ 1 and 1∶ 2, respectively.

Key words: Cellulomonas uda, Trichoderma viride, Mixing ratio, Cellulase, Residues of mushroom substrates

Xie Xiaolin, Gu Zhenhong, Chen Meibiao, Yao Qing, Zhu Honghui. Study on the Related Enzyme Activities in the Residues of Different Mushroom Substrates Inoculated with Cellulomonas uda and Trichoderma viride[J]. Biotechnology Bulletin, 2013, 0(12): 162-166.

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