Identification and Enzymatic Properties of Strain YB Simultaneously Secreting Highly Efficient Cellulase and Xylanase

doi:10.13560/j.cnki.biotech.bull.1985.2019-0497

Abstract

Abstract: The objective of this work is to screen and identify fungi that can degrade lignocellulose and to study its enzyme production characteristics. The Congo red plate coating method was used to screen the fungi with ability of degrading lignocellulose. Then the ITS-rDNA sequence analysis was combined to identify the enzyme production conditions,and the DEAE Sepharose Fast Flow anion exchange and Chromatography and Sephadex G-100 gel chromatography were employed to separate and purify the crude enzyme solution precipitated by ammonium sulfate,and finally the enzymatic properties were studied. A strain YB degrading lignocellulose was screened and identified as Trichoderma virens. The maximum viability of cellulase and xylanase during fermentation was 313.53±26.78 U/mL and 18120.87±500.37 U/mL,respectively. The cellulase（CMC enzyme）Ib,IV and xylanase Ia were isolated and purified,and their molecular weights were 58.5 kD,22.8 kD and 44.5 kD,respectively,as determined by SDS-PAGE. The optimal enzymatic reaction conditions for the three enzymes were：50℃,pH5.0. Among them,xylanase effectively degraded corncob xylan into xylose and various xylooligosaccharides. In conclusion,the strain Trichoderma virens YB can secrete lignocellulose efficiently degrading enzyme and thus has potential for application in xylanase and xylooligosaccharide production.

Key words: Trichoderma virens, cellulase, xylanase, zymogram

YANG Bin, LI Xiao-bo, ZHOU Lin, OU Pei-yu, JIN Xiao-bao. Identification and Enzymatic Properties of Strain YB Simultaneously Secreting Highly Efficient Cellulase and Xylanase[J]. Biotechnology Bulletin, 2020, 36(2): 110-118.

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