Screening and Identification of Cellulase-producing Strains Isolated from Crayfish Intestine

doi:10.13560/j.cnki.biotech.bull.1985.2019-0789

Abstract

Abstract: The intestinal microbes are the main driving force for crayfish to degrade cellulose and hemicellulose. In order to explore the probiotic resources and genetic resources of degrading cellulose in the crayfish intestine,the cellulase-producing bacteria were isolated from crayfish intestine by plate screening method,and the genome of the maximum active strain was analyzed by Illumina MiSeq high-throughput sequencing. Morphological and 16S rRNA gene molecular identification showed that strain 13 was Bacillus velezensis,and strain 33 was Bacillus subtilis. Strain 33 has multiple hydrolase activities and high cellulose-degrading ability. The high-throughput sequencing results indicated that the bacterial genome contained many carbohydrate-related enzyme genes. Crayfish intestines are rich in cellulose-degrading bacteria,and Bacillus bacteria accounts for a certain proportion.

Key words: crayfish intestine, endophytic bacterium, cellulase, screening and identification, high-throughput sequencing

FENG Guang-zhi, SHI Hui, LIU Bo, WU Yu-ting, WANG Yue-lin, SHI Yu. Screening and Identification of Cellulase-producing Strains Isolated from Crayfish Intestine[J]. Biotechnology Bulletin, 2020, 36(2): 65-70.

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