Analysis of Structure and B Cell Epitope Predicition of Mycoplasmaovipneumoniae DnaK Protein

Abstract

Abstract: The study is based on the amino acid sequence of heat shock protein Hsp70(DnaK protein)of Mycoplasma ovipneumoniae(Mo)separatated in guizhou province, using bioinformatics methods—DNA Rtar software and online server bioinformatics analysis tools, through the Jameson-Wolf method, Kyte-Doolittle method, Emini method, Welling method and Karplus-Schulz method respectively forecast the parameters, such as antigenic index, hydrophilic, flexibility and surface polarity, and comprehensive analysis, forecasts the B-cell epitopes of the protein, at the same time protein secondary structure prediction and simulation of 3D structure. Results showed that Hsp70 protein of Mo has a low homology with other mycoplasma which can make the sheep disease, explain the protein has good specificity. Mo Hsp 70 protein is an good antigenicity, presents the rules of spatial structure, while C-Terminal(394-598 aa)is more stable and largest region, might be advantage B-cell epitope regions.

Key words: Mycoplasma ovipneumoniae, DnaK protein, Protein structure, B cell epitope

Wang Hui, Luo Chengbo, He Dao, Kong Lingping, Zhou Bijun, Wen Ming, Cheng Zhentao, Wang Kaigong. Analysis of Structure and B Cell Epitope Predicition of Mycoplasmaovipneumoniae DnaK Protein[J]. Biotechnology Bulletin, 2014, 0(3): 159-164.

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