Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (10): 139-143.

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Cloning,Sequence and Tissue Expression Pattern Analysis of HOXC10 Gene in Multi-costa Properties Yaks of Jinchuan

Zhang Yanan1, Xiong Xianrong1, Lan Daoliang1, Fu Mei1, Zhang Yan1, Hou Dingchao2, Ma Dingmei2, Li Shanrong2, Li Jian1   

  1. 1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041; 2. Animal Husbandry and Veterinary Bureau of Jinchuan County, Jinchuan 624100
  • Received:2014-04-22 Online:2014-10-20 Published:2014-10-17

Abstract: The present study aimed at cloning yak HOXC10 gene, and further analysis of the gene structure and function, and reveals the tissue specificity expression pattern. The Jinchuan multi-costa properties yak as materials, this study based on the GenBank published Ovis aries HOXC10 gene sequences for primier designing, using RT-PCR technology to amplification the cDNA full length of HOXC10 gene, and its bioinformatic analysis and expression profiling in various tissues. The sequencing result showed that the length of yak HOXC10 gene is 1 272 bp and contains a 1 029 bp open reading frame(ORF), encoding 342 amino acids. The results of homology analysis showed that yak and bos taurus has the higher similarity(93.2%);Followed by predicting HOXC10 protein molecular weight 38.2 kD, the theory of isoelectric point is
8.45. Evolutionary tree analysis results showed that yak was closest relative with bos taurus, and in the same branch. Tissue expression profile analysis showed that HOXC10 gene is expressed in different degrees in various organizations, which had the highest expression in the liver, the lowest expression in the stomach.

Key words: Yak, Cloning, HOXC10, Sequence analysis, Tissue expression