Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (10): 196-200.

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Analysis of Bacterial Community Structure and Diversity in Wild Ophicordyceps sinensis

Li Hui1, Liu Yang1, Bai Feirong1, Yao Su1, 2, Zahi Nagi2, Cheng Chi1   

  1. 1. Chinese Research Institute of Food and Fermentation Industries Chinese industrial culture collection center,Beijing 100015; 2. Yushu Tibetan Autonomous Prefecture of Sanjiang Source Pharmaceutical Co Ltd,Yushu 810003
  • Received:2014-03-05 Online:2014-10-20 Published:2014-10-17

Abstract: The bacterial community structure of eight wild Ophicordyceps sinensis were investigated using PCR-DGGE.DNA sequencing was proceeded to obtain dominant bacterial population information in different samples. The result of DGGE profile showed that all the samlples contained abundant bacterial community, different samples have some differences among the Shannon-winner index, Richess and evenness, significant differences were observed between samples from distinct locations. Flavihumibacter petaseus, Sphingomonas aestuarii, Geobacillus pallidus and Methylovirgula ligni were commonly detected in all samples, which laid a foundation for further studying on the roles of bacteria during the formation process of Ophicordyceps sinensis.

Key words: Ophicordyceps sinensis, 16S rRNA, V3, DGGE, Bacterial community