Investigation of Bacillus Resources in the Soil of Agroforestry Biome：Case Study in Some Parts of Sichuan and Chongqing

doi:10.13560/j.cnki.biotech.bull.1985.2018-0795

Abstract

Abstract: To provide a foundation for new resource mining of the Bacillus-like species and development of microbial agents,we investigated the species distribution and diversity of Bacillus-like isolates from soil samples in the agroforestry biome of Sichuan province and Chongqing city. Isolates Bacillus were obtained from the soil samples of agroforestry biome by cultural method,and identified based on 16S rRNA gene sequences. The community structure of the Bacillus-like bacteria was analyzed using the diversity index. A total of 95 Bacillus-like isolates were obtained from the 5 soil samples,and identified as 38 species belonging to 7 genera within Bacillaceae by 16S rRNA gene sequences. The species number of each genus was 22 for Bacillus,4 for Lysinibacillus,7 for Paenibacillus,2 for Brevibacillus,and 1 for Psychrobacillus,Rummeliibacillus and Fictibacillus respectively. And 16S rRNA similarities between 6 isolates and their most-closed type species were lower than 98.65 % of species defined threshold,indicating that these 6 isolates might be potential new Bacillus species. The order of Shannon index of Bacillus from the agroforestry biome was as forest land in Leshan > farmland in Yanting County > forest land in Zhong County > forest land Yanting County,respectively. According to Bacillus-like species and content,as Euclidean distance was about 15,Bacillus-like species could be divided into 2 clusters,i.e. high-content wide-distribution and low-content uneven-distribution. Thus,there are rich Bacillus-like species in the agroforestry biome,which offers the guarantees for the development and utilization of Bacillus-like resources.

Key words: Bacillus, agroforestry biome, diversity, 16S rRNA gene

LIU Guo-hong, LIU Qin-ying, LIU Bo, CHE Jian-mei, CHEN Qian-qian. Investigation of Bacillus Resources in the Soil of Agroforestry Biome：Case Study in Some Parts of Sichuan and Chongqing[J]. Biotechnology Bulletin, 2019, 35(3): 78-86.

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