Analysis of Differential Metabolites and Bacterial Community Structure in Soils of a Pineapple Orchard in Different Continuous-cropping Years

doi:10.13560/j.cnki.biotech.bull.1985.2021-0273

Abstract

Abstract:

In order to investigate the effects of continuous-cropping pineapple on the soil metabolites and bacteria community structure,taking the soil of uncultivated land（T0）on the edge of the orchard as the control,untargeted metabolomics and bacterial 16S high-throughput sequencing were combined to compare and analyze the metabolites of the soils continuous-cropping pineapple for 5（T5）and 15（T15）years respectively as well as differences in bacterial community. The results of metabolites analysis indicated that there were totally 120 and 80 differential metabolites in the soil of T5 and T15 when compared with T0. And 11 significantly different metabolites（VIP>1,P<0.05）were detected between T0-T5 group and T0-T15 group in total,including 4 organic acids,3 carbohydrates,2 amino acid derivatives,2 alcohols. KEGG metabolic pathways analysis suggested that the differential metabolites were mostly enriched in 11 key metabolic pathways,e.g.,biosynthesis of unsaturated fatty acids. The bacterial 16S high-throughput sequencing profiling indicated that the bacteria diversity and abundance of the soil in T5 increased when compared with T0. The bacteria diversity increased while the abundance decreased in T15 soil when compared with T0. And there were significant differences in bacterial community structure between T5 and T15. In the levels of phyla and genus,the relative abundances of soil bacteria in T5 and T15 were significantly higher than that in T0. The proportion of two dominant bacteria communities in the soil of the pineapple orchard,Proteobacteria and Acidobacteriota,increased in T5 but decreased in T15 when compared with T0. CCA analysis showed that ccontinuous-cropping years were in significantly negative correlation with pH. This work would contribute to provide a reference for related research on the impacts of ccontinuous-cropping pineapple on the soil environment and contribute to provide the guidance for improving soil management level of pineapple orchard.

Key words: continuous-cropping pineapple, soil, differential metabolites, bacterial community structure

LIU Chuan-he, HE Han, HE Xiu-gu, LIU Kai, SHAO Xue-hua, LAI Duo, KUANG Shi-zi, XIAO Wei-qiang. Analysis of Differential Metabolites and Bacterial Community Structure in Soils of a Pineapple Orchard in Different Continuous-cropping Years[J]. Biotechnology Bulletin, 2021, 37(8): 162-175.

Figures/Tables 12

References 43

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项目Item	参数Parameter
进样量Sample volume	1 μL
分流模式Front inlet mode	不分流模式Splitless mode
隔垫吹扫流速 Front inlet septum purge flow	3 mL/min
载气Carrier gas	氦Helium
色谱柱Column	DB-5MS（30 m×250 μm×0.25 μm）
柱流速Column flow	1 mL/min
柱箱升温程序Oven temperature ramp	50℃保持1 min,以10℃/min的速度升至 310℃,并保持8 min 50℃ Stay at 50℃ for 1 min,raise to 310℃ at a rate of 10℃/min,Stay at 50℃ for 8 min
前进样口温度 Front injection temperature	280℃
传输线温度 Transfer line temperature	280℃
离子源温度 Ion source temperature	250℃
电离电压Electron energy	-70 eV
质量范围Mass range	m/z：50-500
扫描速率Acquisition rate	12.5光谱/秒Spectra per second
溶剂延迟Solvent delay	6.35 min

项目Item	参数Parameter
进样量Sample volume	1 μL
分流模式Front inlet mode	不分流模式Splitless mode
隔垫吹扫流速 Front inlet septum purge flow	3 mL/min
载气Carrier gas	氦Helium
色谱柱Column	DB-5MS（30 m×250 μm×0.25 μm）
柱流速Column flow	1 mL/min
柱箱升温程序Oven temperature ramp	50℃保持1 min,以10℃/min的速度升至 310℃,并保持8 min 50℃ Stay at 50℃ for 1 min,raise to 310℃ at a rate of 10℃/min,Stay at 50℃ for 8 min
前进样口温度 Front injection temperature	280℃
传输线温度 Transfer line temperature	280℃
离子源温度 Ion source temperature	250℃
电离电压Electron energy	-70 eV
质量范围Mass range	m/z：50-500
扫描速率Acquisition rate	12.5光谱/秒Spectra per second
溶剂延迟Solvent delay	6.35 min

代谢物序号 Metabolite ID	代谢物名称 Metabolite name	分类Classification	表达差异倍数 log₂Fold Change		VIP值 VIP value
代谢物序号 Metabolite ID	代谢物名称 Metabolite name	分类Classification	T0-T5	T0-T15	T0-T5	T0-T15
meta_395	（2R,3S）-2-羟基-3-异丙基丁二酸（2R,3S）-2-hydroxy-3-isopro pylbutanedioic acid	有机酸Organic acid	1.924	2.099	1.504	1.685
meta_423	4-羟基苯甲酸4-Hydroxybenzoic acid	有机酸Organic acid	1.538	2.191	1.524	1.635
meta_465	4-羟基-3-甲氧基苯甲酸 4-hydroxy-3-methoxybenzoic acid	有机酸Organic acid	1.569	1.879	1.496	1.665
meta_484	3,4-二羟基苯甲酸3,4-dihydroxybenzoic acid	有机酸Organic acid	1.740	1.746	1.430	1.642
meta_304	N-乙酰基-β-丙氨酸N-Acetyl-beta-alanine	氨基酸衍生物 Amino acid derivatives	2.211	3.186	1.491	1.726
meta_489	N-氨基甲酰谷氨酸N-Carbamylglutamate	氨基酸衍生物 Amino acid derivatives	2.327	3.440	1.506	1.686
meta_504	葡萄糖Glucose	糖类Carbohydrates	4.878	1.580	2.840	1.634
meta_706	槐糖Sophorose	糖类Carbohydrates	4.201	-3.892	1.471	1.112
meta_789	松三糖Melezitose	糖类Carbohydrates	1.292	-1.386	1.368	1.156
meta_783	（3β,5α,6β）-胆固醇3,5,6-三醇（3β,5α,6β）-Cholestane-3,5,6-triol	醇类Alcohols	3.071	1.568	1.428	1.469
meta_513	山梨糖醇Sorbitol	醇类Alcohols	2.244	1.901	1.493	1.607

代谢物序号 Metabolite ID	代谢物名称 Metabolite name	分类Classification	表达差异倍数 log₂Fold Change		VIP值 VIP value
代谢物序号 Metabolite ID	代谢物名称 Metabolite name	分类Classification	T0-T5	T0-T15	T0-T5	T0-T15
meta_395	（2R,3S）-2-羟基-3-异丙基丁二酸（2R,3S）-2-hydroxy-3-isopro pylbutanedioic acid	有机酸Organic acid	1.924	2.099	1.504	1.685
meta_423	4-羟基苯甲酸4-Hydroxybenzoic acid	有机酸Organic acid	1.538	2.191	1.524	1.635
meta_465	4-羟基-3-甲氧基苯甲酸 4-hydroxy-3-methoxybenzoic acid	有机酸Organic acid	1.569	1.879	1.496	1.665
meta_484	3,4-二羟基苯甲酸3,4-dihydroxybenzoic acid	有机酸Organic acid	1.740	1.746	1.430	1.642
meta_304	N-乙酰基-β-丙氨酸N-Acetyl-beta-alanine	氨基酸衍生物 Amino acid derivatives	2.211	3.186	1.491	1.726
meta_489	N-氨基甲酰谷氨酸N-Carbamylglutamate	氨基酸衍生物 Amino acid derivatives	2.327	3.440	1.506	1.686
meta_504	葡萄糖Glucose	糖类Carbohydrates	4.878	1.580	2.840	1.634
meta_706	槐糖Sophorose	糖类Carbohydrates	4.201	-3.892	1.471	1.112
meta_789	松三糖Melezitose	糖类Carbohydrates	1.292	-1.386	1.368	1.156
meta_783	（3β,5α,6β）-胆固醇3,5,6-三醇（3β,5α,6β）-Cholestane-3,5,6-triol	醇类Alcohols	3.071	1.568	1.428	1.469
meta_513	山梨糖醇Sorbitol	醇类Alcohols	2.244	1.901	1.493	1.607

代谢通路ID Metabolic pathway ID	代谢通路名称 Pathway name	-lgp		代谢物序号 Metabolite ID
代谢通路ID Metabolic pathway ID	代谢通路名称 Pathway name	T0-T5	T0-T15	代谢物序号 Metabolite ID
ko00051	果糖和甘露糖代谢 Fructose and mannose metabolism	5.5963	4.6088	山梨糖醇meta_513 Sorbitol meta_513
ko00100	类固醇生物合成 Steroid biosynthesis	2.3240	7.2183	胆固醇meta_762 Cholesterol meta_762
ko00940	苯丙烷生物合成 Phenylpropanoid biosynthesis	3.3804	3.2558	4-羟基肉桂酸meta_517 4-Hydroxycinnamic acid meta_517
ko00130	泛醌和其他萜类醌的生物合成 Ubiquinone and other terpenoid-quinone biosynthesis	3.3901	3.8455	4-羟基苯甲酸meta_423;4-羟基肉桂酸meta_517 4-Hydroxybenzoic acid meta_423;4-Hydroxycinnamic acid meta_517
ko00350	酪氨酸代谢 Tyrosine metabolism	3.3733	1.6328	4-羟基肉桂酸meta_517 4-Hydroxycinnamic acid meta_517
ko00360	苯丙氨酸代谢 Phenylalanine metabolism	2.7754	2.7181	4-羟基苯甲酸meta_423;4-羟基肉桂酸meta_517 4-Hydroxybenzoic acid meta_423;4-Hydroxycinnamic acid meta_517
ko00400	苯丙氨酸,酪氨酸和色氨酸的生物合成 Phenylalanine,tyrosine and tryptophan biosynthesis	3.4807	1.9702	3,4-二羟基苯甲酸meta_484 3,4-Dihydroxybenzoic acid meta_484
ko00591	亚油酸代谢 Linoleic acid metabolism	2.3376	4.1084	亚油酸meta_594 Linoleic acid meta_594
ko00790	叶酸生物合成 Folate biosynthesis	6.7045	3.6467	4-羟基苯甲酸meta_423 4-Hydroxybenzoic acid meta_423
ko00950	异喹啉生物碱的生物合成 Isoquinoline alkaloid biosynthesis	3.0892	4.2224	4-羟基肉桂酸meta_517 4-Hydroxycinnamic acid meta_517
ko01040	不饱和脂肪酸的生物合成 Biosynthesis of unsaturated fatty acids	2.7263	2.9874	亚油酸meta_594;山嵛酸meta_681;木质酸meta_714 Linoleic acid meta_594. Behenic acid meta_681.Lignoceric acid meta_714