Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (11): 147-151.

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Cloning and Sequence Analysis of MyoD Gene in Red Tilmpa Oreochromis sp.(O.mossambicus×O.niloticus)

Guo Yihui ,Fan Sigang, Huang Guiju, Zhu Kecheng, Yu Dahui   

  1. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300
  • Received:2014-04-16 Online:2014-11-07 Published:2014-11-07

Abstract: As a member of myogenic regulatory factors(MRFs), MyoD play a positive regulation in the process of muscle proliferating.In this study, MyoD cDNA of Red Tilmpa Oreochromis sp.(O.mossambicus×O.niloticus)was obtained using homology cloning strategy and rapid cDNA end amploification. The results showed that the open reading frame(ORF)of MyoD was 903 bp length and encoded 300 amino acids, including basic helix-loop-helix(bHLH)domain which composed of 1st to 114th amino acids and 109th to 165th amino acids, CDK4 combined structure(199th to 213th)and Helix III structure(233th to 249th). The signal peptide was not detected in MyoD. The secondary structure of MyoD protein is HLH dimmer. The phylogenetic trees was constructed based on some fish MyoD ORF and suggested that Oreochromis sp. O. aureaus and O. niloticus were clustered together firstly.

Key words: MyoD , Sequence analysis , Cloning , Red Tilmpa(Oreochromis sp.)