Detection of Quorum Sensing in Vibrio parahaemolyticus Isolated from Macrobrachium rosenbergii

Abstract

Abstract: Vibrio parahaemolyticus VIB461 and VIB800, which were isolated from Macrobrachium rosenbergii, were found to produce three kinds of quorum sensing signals using three different quorum sensing reporter strains V.harveyi JMH612, V.harveyi JMH597 and V.harveyi JAF375, and LuxS gene of the two strains were also amplified by PCR. The results showed that both strains produced three kinds of singnal molecules:HAI-1, AI-2 and CAI-1. The level of three signal molecules all ascended with an increase in bacterial population density, reached high levels during the late exponential phase or early stationary phase, and decreased afterwards. The PCR amplification sequences both shared more than 95% similarity with the reported LuxS gene of V. Parahaemolyticus, which indicated that both strains harbored LuxS gene.

Key words: Vibrio parahaemolyticus, Quorum sensing, HAI-1, AI-2, LuxS, gene CAI-1

Wang Ying, Zhu Suqin, Zhang Caili, Zeng Mingyong. Detection of Quorum Sensing in Vibrio parahaemolyticus Isolated from Macrobrachium rosenbergii[J]. Biotechnology Bulletin, 2014, 0(3): 146-150.

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