Selection, Identification and Characteristics of a Cucumber Growth-Promotion Strain of Rhizobacteria

Abstract

Abstract: Salkowski colorimetric was used to evaluate IAA metabolism of different strains, the use of plates and pot trials were conducted to detect that strains improve plant viability；strain was identified by the method of combination strain’ Physiological -biochemical character and 16S rRNA sequence, and further the influence of different carbon sources and nitrogen source in medium on metabolism ability of IAA was explored. Results showed that eight strains that could excrete IAA were isolated from cucumber roots. Strain SGM7 was the highest in IAA excreting capability(23. 59 mg/L in 48 h of cultivation). Based on its morphological feature, Some of its physiological and biochemical characteristics and its 16S rRNA sequence analysis, it was identified as Bacillus Pumilus. The optimum fermentation medium contained 1% of protein, 2% of maize powder, 0. 25% of wheat bran, 0. 05% of ammonium sulfate, and 0. 05% of potassium nitrate. Strain SGM7 had the highest in IAA excreting capability(35. 87 mg/L in 24 h of cultivation).

Key words: PGPR, Selection and identification, IAA, Orthogonal design

Ge Chunhui, Meng Ajing, Ma Yanru, Yang Xinhua, Wang Xinyong, Sun Jiusheng. Selection, Identification and Characteristics of a Cucumber Growth-Promotion Strain of Rhizobacteria[J]. Biotechnology Bulletin, 2014, 0(3): 94-99.

References

[1] 花东来, 欧秀玲, 祝建波, 等. PGPR菌液处理对新疆加工番茄种子萌发的影响[J]. 新疆农业科学, 2013, 50(3):484-489.
[2] Mendes R, Pizzirani-Kleiner AA, Araujo WL, et al. Diversity of cultivated endophytic bacteria from sugarcane:genetic and biochemical characterization of Burkholderia cepacia complex isolates[J]. Appl Environ Microbiol, 2007, 73:7259-7267.
[3] Kuklinsky-Sobral J, Araújo WL, et al. Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion[J]. Environ Microbiol, 2004, 6:1244-1251.
[4] Rajaee S, Alikhani HA, Raiesi F. Effect of plant growth promoting potentials of Azotobacter chroococcum native strains on growth, yield and uptake of nutrients in wheat[J]. Journal of Science and Tech-nology of Agriculture and Natural Resources Water and Soil Science, 2007, 11(41):285-297.
[5] 陈波, 丁延芹, 马海林, 等. 樱桃根际促生细菌的筛选与鉴定[J]. 微生物学通报, 2012, 39(12):1746-1754.
[6] 李引, 虞丽, 李辉信, 等. 一株花生根际促生菌的筛选鉴定及其特性研究[J]. 生态与农村环境学报, 2012, 28(4):416-421.
[7] 刘琳, 孙磊, 张瑞英, 等. 春兰根中可分泌吲哚乙酸的内生细菌多样性[J]. 生物多样性, 2010, 18(2):195-200.
[8] 布坎南RE, 吉本斯?伯杰. 细菌鉴定手册[M]. 1984, 北京:科学出版社.
[9] 康贻军 , 程洁, 梅丽娟, 等. 植物根际促生菌的筛选及鉴定[J]. 微生物学报, 2010, 50(7):853-861.
[10] 方芳, 刘佳莉, 史煦涵, 等. 石油污染土壤中植物根际促生菌的筛选及特性分析[J]. 生物技术通报, 2012(6):154-158.
[11] Arshad M, Frankenberger WT. Microbial production of plant hormones[J]. Plant and Soil, 1991, 133:1-8.
[12] Sarwar M, Frankenberger WT. Tryptophan dependent biosynthesis of auxins in soil[J]. Plant and Soil, 1994, 160:97-104.
[13] Tsavkelova EA, Cherdyntseva TA, Botina SG, Netrusov AI. Bacteria associated with orchid roots and microbial production of auxin[J]. Microbiological Research, 2007, 162:69-76.
[14] 康贻军, 程洁, 梅丽娟, 等. 植物根际促生菌的筛选及鉴定[J]. 微生物学报, 2010, 50(7):853-861.
[15] Spaepen S, Vanderleyden J, Remans R. Indole-3-acetic acid in mic-robial and microorganism-plant signaling[J]. FEMS Microbiol Rev, 2007, 31:425-448.
[16] 何志刚, 王秀娟, 董环, 等. PGPR菌肥对马铃薯产量与肥料利用率影响的初步研究[J]. 中国土壤与肥料, 2013, 2:100-103.
[17] 段秀梅, 高晓蓉, 吕军, 等. 两株土壤分离菌的解磷能力及对玉米的促生作用[J]. 中国土壤与肥料, 2010(2):79-85.
[18] Zemrany HE, Czarnes S, Hallett PD, et al. Early changes in root characteristics of maize(Zea mays)following seed inoculation with the PGPR Azospirllum lipoferum CRT1[J]. Plant and Soil, 2007, 291(1/2):109-118.
[19] Chanway CP, Holl FB. First year field performance of spruce seed-lings inoculated with plant growth promoting rhizobacteria[J]. Canadian J Microbiol, 1993, 39(11):1084-1088.
[20] Ahmad F, Ahmad I, Khan MS. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities[J]. Microbiological Research, 2008, 163(2):173-181.
[21] Cattelan AJ, Hartel PG, Fuhrmann JJ. Screening for plant growth-promoting rhizobacteria to promote early soybean growth[J]. Soil Science Society of America Journal, 1999, 63:1670-1680.
[22] 郑元元, 岳海涛, 石在强, 等. 盐胁迫下解盐促生细菌Rs-5和Rs-198促进棉花种子发芽的机理探讨[J]. 中国农业科学, 2008, 41(5):1326-1332.
[23] Datta C, Basu PS. Indole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub[J]. Microbiol Res, 2000, 155:123-127.
[24] Sridevi M, Mallaiah KV. Production of indole-3-acetic acid byRhizobium isolates from sesbania species[J]. Afr J Micro-biol Res, 2007, 1(7):125-128.
[25] Ahmad F, Ahmad I, Khan MS. Indole acetic acid pro-duction by the indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and absence of tryptophan[J]. Turk J Biol, 2005, 29:29-34.
[26] Shokri D, EmtiaziG. Indole-3-acetic acid(IAA)production in symbiotic and non-symbiotic nitrogen-fixing bacteria and its optimization by Taguchi design[J]. Curr Microbiol, 2010, 61:217-225.