Isolation and Characterization of Endophytic Strains Producing Exopolysaccharide

Abstract

Abstract: In this study, 24 strains were isolated from carrot, aloe, potato, sweet potato and purple potato. As screening by phenol-sulfuric acid assay, three strains, 25-Z-1, 25-Z-3 and 12-L-6, were proved to be as high exopolysaccharide producing strains. The results of 16S rDNA sequence determination showed that 25-Z-1 and 25-Z-3 isolated from purple potato was about 100% identity compared with Bacillus pumilus strain ST277 and 99% identity compared with Bacillus cereus strain Se05, respectively. Meanwhile the 12-L-6 isolated from aloe identified as Bacillus sp. GZT, with the similarity is of 99%. Culture in LB medium, 30℃ and 170 r/min for 7 d, yield of EPS could reach to 30.3 mg/L, 29.1 mg/L and 28.2 mg/L, respectively.

Key words: Endophyte, Exopolysaccharide, 16S, rDNA

Huang Yicheng, Liu Yang, Pang Xin, Ma Zhongrui, Han Donglei, Chen Min. Isolation and Characterization of Endophytic Strains Producing Exopolysaccharide[J]. Biotechnology Bulletin, 2014, 0(4): 147-151.

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