Optimization of Submerged Culture Requirements for the Cellulase Activity by Phlebiopsis gigantea and Screening the Most Effective Strain

Abstract

Abstract: The activity of extracellular cellulase could be used for screening the effective biocontrol strain. We could acquire the most effective Phlebiopsis gigantea isolate associated with biocontrol conifer root and butt rots by determining cellulase activity of them. Based on single factor and orthogonal experiment, the optimum medium compositions for the highest cellulase activity were(g/L)：glucose 30.00, peptone 5.00, KH₂PO₄3.00, MgSO₄?7H₂O 1.50, the optimal liquid medium volume was 120 mL/250 mL, the optimal inoculum volume was 5%(V/V), initial pH4.0. By determining cellulase activity of P. gigantea isolates, we concluded that P. gigantea 08077 had the highest cellulase activity and 13025 was the lowest. P. gigantea 08077 is better to control the Chinese Heterobasidion parviporum than Rotstop-F that had been registered in Finland.

Key words: Phlebiopsis gigantea , Submerged culture, Optimization, Cellulase activity, Orthogonal array design

Li Xingchun, He Shuanghui, Dai Yucheng. Optimization of Submerged Culture Requirements for the Cellulase Activity by Phlebiopsis gigantea and Screening the Most Effective Strain[J]. Biotechnology Bulletin, 2014, 0(4): 152-158.

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