Screening，Identification and Characterization of an Acid q-amylase Producing Strain and Optimization of Its Fermentable Condition

Abstract

Abstract: Multiple new acid α-amylase producing strains were isolated from soil which is rich in starch. A strain which has the highest enzyme activity was identified as Bacillus subtilis A-7 through analysis of 16S rDNA gene. The optimum temperature and pH was 60℃ and 4.5. The single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium. The composition of the best medium was 2% soluble starch, 2% peptone, 0.07% CaCl₂, 0.8% Na₂HPO₄. Under this optimal condition, the activity of acid α-amylase was 221 U/mL and 2.3-fold more than that of no optimization.

Key words: Acid α-amylase 16S, rDNA, Bacillus subtilis, Optimum temperature, Optimum pH, Orthogonal experiment

Wang Jianling, Chen Zhixin, Liu Yihan, Lu Fuping. Screening，Identification and Characterization of an Acid q-amylase Producing Strain and Optimization of Its Fermentable Condition[J]. Biotechnology Bulletin, 2014, 0(4): 159-163.

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