Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (4): 169-175.

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The Study of the Structure of Perchlorate(ClO4-)-degrading Bacterial Communities Under Autotrophic Conditions

Xie Yuxuan1, Guan Xiangyu2, Yu Lisha1, Liu Fei1,   

  1. (1. School of Water Resources and Environment,China University of Geosciences,Beijing 100083;
    2. School of Ocean Sciences,China University of Geosciences,Beijing 100083)
  • Received:2013-10-31 Online:2014-04-29 Published:2014-04-29

Abstract: For the purpose of further investigating the biological degradation under an autotrophic condition and well understanding the microbial community structures in a complex environment, hydrogen was used as an electron donor to completely reduce perchlorate(ClO4-)in this study. The composition of microbial communities after degradation was analyzed via the construction of a cloning library by using the High-Throughput Sequencing method(HiSeq 2000). 71 days were needed to completely degrade 10 mg/L ClO4-. Microbial phylogenic analysis of HD(hydrogen degradation)after degradation indicated that the relative abundance of total bacteria in the HD was 84.96% whereas the relative abundance of Proteobacteria was 68.11%, whose percentage accounting for the total bacteria reached to 80.16%. The relative abundance of Dechloromonas which is representative in PRB was 2.7% in the HD. Simultaneously, the relative abundance of Azospira was 3.1%. KEGG was used to analyze the function of bacteria in HD. The relative abundance of genes which engaged in carbohydrate metabolism was 4.75%, and the genes included in energy metabolism was 3.35%, whereas the genes participated in nitrogen cycle was 0.72%, and the genes involving chloride transformation was 0.83%. It was demonstrated that degradation of ClO4- in a complicated condition was achieved by various kinds of microbes rather than a single one. Adding hydrogen as an electron donor to change microbial community played a role in the purification or selection process in the system, which allowed the complex systems to have the specific capacity to remove given contaminates.

Key words: Perchlorate(ClO4-), Electron donor, Hydrogen, The composition of microbial communities, High-Throughput Sequencing