Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (5): 45-51.

• Technology and methods • Previous Articles     Next Articles

Research Progress of Improving the Actinomycetes Secondary Metabolite Production Method

Lei Xiuqing Li Li Huang Jianzhong   

  1. (Engineering Research Center of Indunstrial Mcrobiology(Ministry of Education),Engineering Research Center of Fujian Modern Fermentation Technology,College of Life Sciences,Fujian Normal Universty,Fuzhou 350108
  • Received:2013-10-16 Online:2014-05-23 Published:2014-05-24

Abstract: Actinomycetes secondary metabolites have been the important sources of developing antibiotics and other drugs. Gene technology has been widely practiced for improving the yield of Actinomycetes secondary metabolites. This review summarized a series of technical methods used for improving Actinomycetes secondary metabolites,consisting of over-expressing the key structural genes and/or positive regulation genes,knocking out the negative regulatory genes,doubling the clusters,mutating ribosomal protein genes,and expressing the foreign cluster heterologously. These methods could be used for new drug discovery and strains improvement.

Key words: Secondary, Netabolites, Ribosome engineering, Heterologous expression, Actinomycetes