Research Progress of Cell-SELEX

Abstract

Abstract: Aptamers have high affinity to target molecules, which are selected by an in vitro process known as cell systematic evolution of ligands by exponential enrichment(Cell-SELEX). SELEX is carried out using purified target molecules, but whole live cells could be used as selection targets in Cell-SELEX progress. The advantage of this technology is that aptamers could be functional with a native conformation of the target molecule on live cells. Cell surface transmembrane proteins would also be the targets. In addition, cell-specific aptamers can be obtained without any knowledge about cell surface molecules on the target cells. In this paper, the progress of Cell-SELEX technology was reviewed.

Key words: Cell-SELEX Biomarkers, Cancer, Research Nucleic acids, Aptamer

Zhao Zhonglin, Li Yan, Yuan Chao. Research Progress of Cell-SELEX[J]. Biotechnology Bulletin, 2014, 0(5): 52-56.

References

[1] Klussmann S. The Aptamer handbook：functional oligonucleotides and their applications[M]. Weinheim：Wiley-VCH, 2006.
[2] Stoltenburg R, Reinemann C, Strehlitz B. SELEX-a(r)evolutionary method to generate high-affinity nucleic acid ligands[J]. Biomol Eng, 2007, 24：381-403.
[3] Ellington A, Szostak JW. In vitro selection of RNA molecules that bind specific ligands[J]. Nature, 1990, 346：818-822.
[4] Tuerk C, Gold L. Systematic evolution of ligands by exponential enr-ichment：RNA ligands to bacteriophage T4 DNA polymerase[J]. Science, 1990, 249：505-510.
[5] Vant-Hull B, Payano-Baez A, Davis RH, et al. The mathematics of SELEX against complex targets[J]. J Mol Biol, 1998, 278：579-597.
[6] Morris KN, Jensen KB, Julin CM, et al. High affinity ligands from in vitro selection：complex targets[J]. Proc Natl Acad Sci USA, 1998, 95：2902-2907.
[7] Homann M, Goringer HU. Combinatorial selection of high affinity RNA ligands to live African trypanosomes[J]. Nucleic Acids Res, 1999, 27：2006-2014.
[8] Lorger M, Engstler M, Homann M, et al. Targeting the variable surface of African trypanosomes with variant surface glycoprotein-specific, serum-stable RNA aptamers[J]. Eukaryot Cell, 2003, 2：84-94.
[9] Ulrich H, Magdesian MH, Alves MJ, et al. In vitro selection of RNA aptamers that bind to cell adhesion receptors of Trypanosoma cruzi and inhibit cell invasion[J]. J Biol Chem, 2002, 277：20756-20762.
[10] Zhang Y, Chen Y, Han D, et al. Aptamers selected 1 by cell-SELEX for application in cancer studies[J]. Bioanalysis, 2010, 2：907-918.
[11] Phillips JA, Lopez-Colon D, Zhu Z, et al. Applications of aptamers in cancer cell biology[J]. Anal Chim Acta, 2008, 621：101-108.
[12] Shangguan D, Li Y, Tang Z, et al. Aptamers evolved fromlive cells as effective molecular probes for cancer study[J]. Proc Natl Acad Sci USA, 2006, 103：11838-11843.
[13] Guo KT, Schafer R, Paul A, et al. Aptamer-based strategies for stem cell research[J]. Mini Rev Med Chem, 2007, 7：701-705.
[14] Iwagawa T, Ohuchi SP, Watanabe S, et al. Selection of RNA aptamers against mouse embryonic stem cells[J]. Biochimie, 2012, 94：250-257.
[15] Tang Z, Parekh P, Turner P, et al. Generating aptamers for recognition of virus-infected cells[J]. Clin Chem, 2009, 55：813-822.
[16] Blank M, Weinschenk T, Priemer M, et al. Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels. Selective targeting of endothelial regulatory protein pigpen[J]. J Biol Chem, 2001, 276：16464-16468.
[17] Berezovski MV, Lechmann M, Musheev MU, et al. Aptamer-facilitated biomarker discovery(AptaBiD)[J]. J Am Chem Soc, 2008, 130：9137-9143.
[18] Shoji O. Cell-SELEX technology[J]. Bio Research Open Access, 2012, 1(6)：265-272.
[19] Farokhzad OC, Jon S, Khademhosseini A, et al. Nanoparticle-aptamer bioconjugates：a new approach for targeting prostate cancer cells[J]. Cancer Res, 2004, 64：7668-7672.
[20] Chu TC, Twu KY, Ellington AD, et al. Aptamer mediated siRNA delivery[J]. Nucleic Acids Res, 2006, 34：e73.
[21] McNamara JO, Andrechek ER, Wang Y, et al. Cell type-specific delivery of siRNAs with aptamer-siRNA chimeras[J]. Nat Biotechnol, 2006, 24：1005-1015.
[22] Wu CC, Castro JE, Motta M, et al. Selection of oligonucleotide aptamers with enhanced uptake and activation of human leukemia B cells[J]. Hum Gene Ther, 2003, 14：849-860.
[23] Mende M, Hopert A, Wunsche W, et al. A hexanucleotide selected for increased cellular uptake in cis contains a highly active CpG-motif in human B cells and primary peripheral blood mononuclear cells[J]. Immunology, 2007, 120：261-272.
[24] Kolesnikova O, Kazakova H, Comte C, et al. Selection of RNA aptamers imported into yeast and human mitochondria[J]. RNA, 2010, 16：926-941.
[25] Mi J, Liu Y, Rabbani ZN, et al. In vivo selection of tumor-targeting RNA motifs[J]. Nat Chem Biol, 2010, 6：22-24.
[26] Douglas SM, Bachelet I, Church GM. A logic-gated nanorobot for targeted transport of molecular payloads[J]. Science, 2012, 335：831-834.
[27] Smith JE, Medley CD, Tang Z, et al. Aptamer-conjugated nanoparticles for the collection and detection of multiple cancer cells[J]. Anal Chem, 2007, 79：3075-3082.
[28] Schafer R, Wiskirchen J, Guo K, et al. Aptamer-based isolation and subsequent imaging of mesenchymal stem cells in ischemic myocard by magnetic resonance imaging[J]. Rofo, 2007, 179：1009-1015.
[29] Guo K, Wendel HP, Scheideler L, et al. Aptamer-based capture molecules as a novel coating strategy to promote cell adhesion[J]. J Cell Mol Med, 2005, 9：731-736.
[30] Guo KT, Schafer R, Paul A, et al. A new technique for the isolation and surface immobilization of mesenchymal stem cells from whole bone marrow using high-specific DNA-aptamers[J]. Stem Cells, 2006, 24：2220-2231.
[31] Schroeder H, Ellinger B, Becker CF, et al. Generation of live-cell microarrays by means of DNA-directed immobilization of specific cell surface ligands[J]. Angew Chem Int Ed Engl, 2007, 46：4180-4183.
[32] Gartner ZJ, Bertozzi CR. Programmed assembly of 3-dimensional microtissues with defined cellular connectivity[J]. Proc Natl Acad Sci USA, 2009, 106：4606-4610.