Abstract: Abstract: Cell-surface display technology was used widely in the filed of high throughput screening of a mutant library, which promoted the development of protein engineering. The carboxyl terminal domain of the EstA from Pseudomonas aeruginosa was used as carrier protein and the lipA gene was fused to the estA’ gene by overlap extension PCR. The fusion gene lipA-estA’ was then inserted the genetically modified plasmid pACYC-Duet, which promoter was changed into lacZ promoter. The resulting plasmid pBCMB-X1 was transformed into E. coli JK321 and E. coli UT5600, respectively. The lipA gene was induced expression by IPTG and the recombinant LipA was functionally displayed on the cell surface of E. coli JK321 and E. coli.UT5600, respectively. The hydrolysis activity of the LipA was（2.8±0.1）U/OD and（2.6±0.06）U/OD, respectively.