Label-free Quantitative Proteomics Analysis of a Wheat Near-isogenic Line Pair with Q Exactive Mass Spectrometer

Abstract

Abstract: In this study, the protein expression of a wheat near-isogenic line pair, Taichung 29*6/Yr10 and Taichung 29, have been compared with label-free quantitative proteomic approach based on liquid chromatography tandem Q Exactive hybrid quadrupole-Orbitrap mass spectrometer. By MASCOT database search, a total of 2 257 proteins were identified from both of the samples, and among of them 1 549 proteins were accurately quantitated and 102 differentially expressed proteins（>2 fold）were detected. Gene ontology（GO）analysis showed that these differential proteins were mainly localized to cell matrix and organelles such as ribosome, and mainly involved in binding and catalytic functions. Among of 102 proteins which mainly take part in metabolic, cellular and stress responses processes, Superoxide dismutase, Methionine aminopeptidase, Lysosomal-β-glucosidase, and Ferritin may play some important roles in Taichung 29*6/Yr10 resistance to Yellow rust.

Key words: Wheat, Yellow rust, Resistance, Proteomics Label-free quantitation, Q, Exactive, Mass, Spectrometer

Huang Yu,Feng Jing,Rao Liqun,Xu Shichang,Pan Yinghong. Label-free Quantitative Proteomics Analysis of a Wheat Near-isogenic Line Pair with Q Exactive Mass Spectrometer[J]. Biotechnology Bulletin, 2014, 0(6): 88-95.

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