Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (7): 119-124.

• Research Report • Previous Articles     Next Articles

Transcriptome Analysis for Quercus liaotungensis Koidz. Based on High-throughput Sequencing Technology

Liu Yulin1, Li Wei1, Zhang Zhixiang2   

  1. 1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083;
    2. College of Nature Conservation, Beijing Forestry University, Beijing 100083
  • Received:2013-12-10 Online:2014-07-15 Published:2014-07-16

Abstract: In this study, Illumina Solexa Hiseq 2000 high-throughput sequencing technology was used to get the comprehensive transcriptome from mixed samples of buds, flowers, leaves and fruits of Quercus liaotungensis. As a result, 3.8 Gb effective data was obtained. After de novo assembly by the software of Trinity, a total of 95 800 unigenes were generated, corresponding to a total of 73.57 Mb with a maximum length, average length and N50 of 11 284 bp, 768 bp and 1 373 bp respectively. Using Blastx against the public databases of Nr and Swiss-Prot with an E-value cut-off of 10-5, 38 163 unigene were not found in any databases with a high homology. According to the KEGG pathway assignment, 67 unigene encoding nine key enzymes which may involve in starch synthesis were identified. In addition, 15 901 potential SSR loci were detected from 13 380 unigene. Of them, dinucleotide repeat and trinucleotide repeat accounted for 98.16% of all.

Key words: Quercus liaotungensis, Illumina Solexa, Transcriptome, Starch, SSR