Cloning and Tissue-specific Expression of TCTP Gene in Inner Mongolia Cashmere Goat (Capra hircus)

Abstract

Abstract: The present study aims at cloning the CDS fragment of Translationally controlled tumor protein（TCTP）gene cDNA in Inner Mongolia Cashmere Goat and analyzing its expression pattern. TCTP gene cDNA was cloned by RT-PCR. The nucleotide sequence was analyzed by BLAST, while amino acid sequence was analyzed by online softwares. The tissue-specific expression pattern of TCTP was analyzed by quantitative RT-PCR. The cloned TCTP gene cDNA was 519 bp in length, including a complete ORF encoding 172 amino acids. The full cDNA nucleotide sequence has an identity from 99% to 95% with Ovis aries, Bos taurus, Sus scrofa, Homo sapiens, Macaca mulatta and Rattus norvegicus. Bioinformatics analysis showed that theoretical molecular mass of encoded protein was 19.6 kD, isoelectric point（pI）was 4.673. It contained a N-glycosylation sites, a protein kinase C phosphorylation sites and three casein kinase Ⅱ phosphorylation sites and most probably is localized in cytoplasm. The results of quantitative RT-PCR showed that TCTP gene is expressed in kidney, muscle, pancreas, liver, testis and brain. The expression was higher in liver, whereas lower in brain.

Key words: Inner Mongolia cashmere goat, Translationally controlled tumor protein（TCTP）, Gene cloning, Expression pattern

Yang Limin, Yao Ruiyuan, Guo Zhixin, Bao Chaogetu, He Qiburi, Zheng Xu, Bao Wenlei, Wang Zhigang. Cloning and Tissue-specific Expression of TCTP Gene in Inner Mongolia Cashmere Goat (Capra hircus)[J]. Biotechnology Bulletin, 2014, 0(7): 125-130.

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