Evaluation of Genetic Diversity in the Onchidium struma from Different Geographical Populations in China by Inter Simple Sequence Repeat（ISSR）

Abstract

Abstract: The genetic diversity of Onchidium struma collected from Yancheng（YC）, Shanghai（SH）, Wenzhou（WZ）, Xiamen（XM）, Shenzhen（SZ）, Hainan（HN）in China, was studied using the inter simple sequence repeat（ISRR）technique. The results indicated that the values of percentage of polymorphic band, Nei’s gene diversity and Shannon’s information index were 54.20%-70.99%, 0.1564-0.2191 and 0.2421-0.3324. According to AMOVA analysis, there were significant（P < 0.001）genetic differences among the six populations, 47.25%was attributed to among populations and the rest（52.75%）to differences within populations. The pairwise Fst =0.4725, gene differentiation coefficient（GST=0.3705）among populations and gene flow（Nm=0.8497）indicated that greater genetic differentiation occurred among the populations. Genetic drift was one of the main factors that affecting the population differentiation.

Key words: Onchidium struma , Geographical population , ISSR, Genetic diversity, Genetic differentiation

Wang Chengnuan, Shen Heding, Zheng Pei. Evaluation of Genetic Diversity in the Onchidium struma from Different Geographical Populations in China by Inter Simple Sequence Repeat（ISSR）[J]. Biotechnology Bulletin, 2014, 0(9): 208-212.

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