Isolation, Expression and Identification of Multifunctional Glucoamylase from Marine Streptomyces olivaceus FXJ7.023

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.024

Abstract

Abstract: SoGA, a predicted glucoamylase encoded by a full-length 1 788 bp gene consists of 595 amino acids was cloned from Streptomyces olivaceus strain FXJ7.023 genomic DNA by PCR. Heterologous expression of SoGA in Escherischia coli strains BL21（DE3） plysS was performed and a fusion protein with molecular weight of 83.22 kD was obtained. The novel recombinant glucoamylase showed multifunctional catalytic activity of hydrolyze carboxymethylcellulose and starch under different temperature and pH.

Key words: streptomycetes, glucoamylase, gene clone, protein expression, enzyme activity

Wang Miao, Li Yuanyuan, Yue Changwu, Shao Meiyun , Lü Yuhong, Bao Yuxin, Huang Ying. Isolation, Expression and Identification of Multifunctional Glucoamylase from Marine Streptomyces olivaceus FXJ7.023[J]. Biotechnology Bulletin, 2015, 31(2): 160-166.

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