Immobilization of Glucoamylase onto the Electrospinning PEI/PVA Composite Nanofiber Membrane via Ionic Adsorption

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.025

Abstract

Abstract: It was to study the physical and chemical properties of immobilization of glucoamylase on the PEI/PVA nanofibers via ion bonding. Polyethyleneimine（PEI）/polyvinyl alcohol（PVA） composite nanofiber membrane was prepared by electrospinning technology, and thermal crosslinking was applied to make it possess water stability. Glucoamylase was immobilized onto the electrospinning PEI/PVA composite nanofiber membrane via ion adsorption. Results showed that fourier transform infrared spectrum（FT-IR） indicated that glucoamylase can be successfully fixed on the surface of the electrospinning PEI/PVA composite nanofiber membrane. Then, the enzymatic properties of immobilized glucoamylase were identified. The results have shown that the optimum reaction temperature of immobilized glucoamylase is 65℃, 6 degrees higher than free glucoamylase, and the applicable pH range of immobilized glucoamylase is wider than free glucoamylase significantly. In addition, immobilized glucoamylase also has good thermal stability, storage stability and reusability. The use of ion adsorption can help the protein molecules easily fixed onto the nanofiber membrane, so this method processes a certain application.

Key words: electrospinning, PEI/PVA composite nanofiber membrane, glucoamylase, ionic adsorption, immobilization

Sui Chunhong, Wang Cheng, Dong Shunfu, Han Liqin. Immobilization of Glucoamylase onto the Electrospinning PEI/PVA Composite Nanofiber Membrane via Ionic Adsorption[J]. Biotechnology Bulletin, 2015, 31(2): 167-172.

References

[1] Slivinski CT, Machado AVL, Iulek J, et al. Biochemical characteris-ation of a glucoamylase from Aspergillus niger produced by solid-state fermentation [J]. Braz Arch Biol Technol, 2011, 54（3）：559-569.
[2] Kumar P, Satyanarayana T. Microbial glucoamylases：characteristics and applications [J]. Crit Rev Biotechnol, 2009, 29（3）：225-255.
[3] Homaei AA, Sariri R, Vianello F, et al. Enzyme immobilization：an update [J]. J Chem Biol, 2013, 6（4）：185-205.
[4] 隋颖.生物酶固定化方法的研究新进展[J].山东化工, 2013, 42（8）：71-72.
[5] 游金坤, 余旭亚, 赵鹏.吸附法固定化酶的研究进展[J].化学工程, 2012, 40（4）：1-5.
[6] 董炎炎, 刘长虹, 马霞.酶固定化载体材料的研究进展[J].上海应用技术学院学报：自然科学版, 2013, 13（4）：295-298.
[7] 高启禹, 徐光翠, 陈红丽, 等.纳米材料固定化酶的研究进展[J].生物技术通报, 2013（6）：20-24.
[8] 代云容, 牛军峰, 殷立峰, 等.静电纺丝纳米纤维膜固定化酶及其应用[J].化学进展, 2010, 22（9）：1808-1817.
[9]Greiner A, Wendorff JH, Yarin AL, et al. Biohybrid nanosystems with polymer nanofibers and nanotubes [J]. Appl Microbiol Biotechnol, 2006, 71（2）：387-393.
[10]王程, 董顺福, 韩丽琴, 等.以高压静电纺PVA纳米纤维膜为载体化学合成法固定葡萄糖淀粉酶[J].化工新型材料, 2013, 41（12）：124-127.
[11]卢艳敏, 崔海信, 崔金辉, 等.磁性纳米颗粒作为基因转染载体的研究[J].生物技术通报, 2012（8）：199-204.
[12]王彦芳, 陈奇, 叶蕊芳, 等.明胶/PEG制备多孔性SiO₂载体及葡萄糖淀粉酶的固定[J].无机化学学报, 2007, 23（11）：1959-1964.
[13]刘洪, 张小云, 曾美霞.微波辐射下双酶催化淀粉水解的动力学研究[J].精细化工, 2010, 27（1）：70-73.
[14]李靖, 陈欢林, 柴红.金属螯合亲和膜吸附分离与纯化溶菌酶的研究（Ⅱ）-不同金属螯合亲和膜对溶菌酶的吸附性能[J].高等学校化学学报, 1999, 20（8）：1322-1327.

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