Ectopic-overexpression of AmHsa32 from Ammopiptanthus mongolicus Promoted Heat Tolerance in Nicotiana benthamiana

doi:10.13560/j.cnki.biotech.bull.1985.2015.05.016

Abstract

Abstract: Hsa32 is a novel heat-shock protein（Hsp）and mainly found in land plants and essential for acquired thermo-tolerance. AmHsa32 as one orthologue of the Hsa32 gene, was isolated from a desert plant, Ammopiptanthus mongolicus（Leguminosae）. In order to verify the function of AmHsa32, we constructed the plant expression vector Pcambia2300-35S-AmHsa32-OCS, and transferred it into Nicotiana benthamiana through the medium of Agrobacterium strain. PCR and Western blotting demonstrated that AmHsa32 had been integrated into the genome of Nicotiana benthamiana. Seed germination and seedling growth analysis indicated that overexpressing AmHsa32 resulted in enhanced tolerance to heat stresse in transgenic Nicotiana benthamiana. Our work suggests that AmHsa32 might be a valuable gene for plant resistant breeding with transgene technology.

Key words: Ammopiptanthus mongolicus, AmHsa32, transgene, thermotolerance

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He Qian, Wang Yanping, Chen Yuzhen, Lu Cunfu. Ectopic-overexpression of AmHsa32 from Ammopiptanthus mongolicus Promoted Heat Tolerance in Nicotiana benthamiana[J]. Biotechnology Bulletin, 2015, 31(5): 100-105.

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Ectopic-overexpression of AmHsa32 from Ammopiptanthus mongolicus Promoted Heat Tolerance in Nicotiana benthamiana

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[1]	LI Jia-le, LIN Sheng-hao, XU Wen-tao. Construction of an Ultra-sensitive Colorimetric Biosensor for Insect Resistance Genes Based on Loop-mediated Isothermal Amplification [J]. Biotechnology Bulletin, 2022, 38(8): 69-76.
[2]	ZHANG Ya-han, ZHU Li-xia, HU Jing, ZHU Ya-jing, ZHANG Xue-jing, CAO Ye-zhong. Opportunities and Challenges of Glyphosate in the Application of Biotechnology Breeding in China [J]. Biotechnology Bulletin, 2022, 38(11): 1-9.
[3]	SHI Xin-yue, SHANG Xiao-yao, ZHOU Ling-fang, ZHANG Tie-jun, CHAO Yue-hui. Cloning and Transformation of MsAP₂ Gene in Medicago sativa [J]. Biotechnology Bulletin, 2021, 37(12): 13-21.
[4]	WANG Qi-wen, LI Pan, Pan Cui-yun, HAN Fen-xia. Effect of Ethylene Glycol on the Expression of Exogenous Genes in Vivo [J]. Biotechnology Bulletin, 2019, 35(4): 64-68.
[5]	LIU Cha, HAN Li-hong, WANG Hai-bo, GAO Yong, TANG Li-zhou. Research Advances on Plant Thaumatin-like Protein Family [J]. Biotechnology Bulletin, 2018, 34(3): 9-17.
[6]	Jing Zhaobin, Lei Yushan, Li Yongwu. Biotechnology and Kiwifruit Breeding in China [J]. Biotechnology Bulletin, 2015, 31(7): 1-10.
[7]	Lu Baorong. Analysis of Fitness Effect and Its Application in Assessing Environmental Risk Caused by Transgene Flow [J]. Biotechnology Bulletin, 2015, 31(4): 7-16.
[8]	Wang Hefei, Liu Dongjun. Transgenic Technology: Establishment of Animal Models and Treatment of Diabetes Mellitus [J]. Biotechnology Bulletin, 2015, 31(10): 89-98.
[9]	Lu Rui, Song Shaozheng, Qi Zhengqiang, Ge Xin, Shao Bin, Cheng Yong. Preparation of Recombinant Human Plasminogen Activator in Rabbit Mammary Gland and Detection of Expressed Products [J]. Biotechnology Bulletin, 2015, 31(10): 216-221.
[10]	Li Ji, Lu Xu, Huang Tiandai, Hua Yuwei, Huang Huasun. Optimization of Digoxigenin Based Southern Blot for Transgenic Hevea brasiliensis Analysis [J]. Biotechnology Bulletin, 2014, 0(8): 76-81.
[11]	Dong Ying, Hu Hongxia, Wang Wei, Tian Zhaohui. Construction of Transgenic Goldfish Based on Tol2 Transposable Element [J]. Biotechnology Bulletin, 2014, 0(5): 122-128.
[12]	Gan Yimei, Zhang Shuzhen, Zeng Fanyun, Feng Cuilian, Yang Benpeng. Advance in Sugarcane Transgenic Breeding [J]. Biotechnology Bulletin, 2013, 0(3): 1-9.
[13]	Chen Sijie, Zhang Hefei, Zhang Cuizhen, Peng Gang. Fluorescence-activated Cell Sorting(FACS)of Fluorescently Tagged Cells from Transgenic Zebrafish Larvae for Gene Expression Analysis [J]. Biotechnology Bulletin, 2013, 0(11): 112-116.
[14]	Liang Hongwei Chen Faju Wang Yubing Li Fenglan, Lu Hai. Expression of Human Glucagon-like Peptide-1 in Transgenic Tobaccos [J]. Biotechnology Bulletin, 2013, 0(1): 139-143.