The Study of Genetic Diversity in Tibetan Pig of Tibet

doi:10.13560/j.cnki.biotech.bull.1985.2015.05.034

Abstract

Abstract: mtDNA D-loop was selected as a marker to determine genetic diversity of the 4 Tibetan pig populations（Nyingchi, Shannan, Chamdo and Shigatse）in Tibet. The results showed A + T content（62.90%）was significantly higher than the G + C content（37.1%）, indicating that Tibetan pigs’ mtDNA D-loop was rich in A and T; there was the presence of a base bias. A total of 20 variable sites and 26 haplotypes were identified in the 435 bp nucleotide sequence of mtDNA D-loop, haplotype diversity（Hd）was 0.705 ± 0.021, the average number of nucleotide differences（k）was 1.231, and nucleotide diversity（Pi）was 0.00283. Among them, Hd, k and Pi were the highest in Chamdo Tibetan pig population, and lowest in Shigatse. In addition, Hap1 and Hap3 were 4 shared haplotypes, revealing that there were 2 common maternal ancestor haplotypes in 4 Tibetan pig populations.

Key words: Tibetan pig, mtDNA D-loop, haplotype, genetic diversity

Guo Yongbo, Cai Yuan. The Study of Genetic Diversity in Tibetan Pig of Tibet[J]. Biotechnology Bulletin, 2015, 31(5): 224-230.

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