Gene Cloning, Bioinformatic Analysis and Preliminary Functional Characterization of Gene Encoding Galactinol Synthase 3 in Jatropha curcas

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.014

Abstract

Abstract: It was to study the roles of soluble sugars in the cold-tolerance of Jatropha curcas and their related applications in genetic engineering. The full length cDNA of J. curcas, a gene member of such enzyme family(JcGS3)was cloned herein, with a size of 1 053 bp covering the entire open reading frame(ORF)of 1 008 bp. This ORF encoded a polypeptide of 335 amino acids, with theoretical molecular weight of 38 kD, pI value of 5.44, and the typical DxD motif. Further promoter analysis of the genomic sequence of JcGS3 indicated the occurrence of TATA-box, CAAT-box, cold responsive elements of CRT/DRE, and ABA responsive elements. In addition, JcGS3 was characterized with a significant effect on improving the cold-tolerance of the recombinant yeast strain.

Key words: Jatropha curcas, galactinol synthase 3, cDNA cloning, bioinformatic analysis, yeast expression

Wang Haibo, Zou Zhurong, Gong Ming. Gene Cloning, Bioinformatic Analysis and Preliminary Functional Characterization of Gene Encoding Galactinol Synthase 3 in Jatropha curcas[J]. Biotechnology Bulletin, 2015, 31(7): 91-100.

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