Identification and Expression Analysis of emx1 from Paralichthys olivaceus

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.023

Abstract

Abstract: To explore the function of the empty spiracles homeobox 1(emx1)gene in the development of Paralichthys olivaceus，the emx1 was cloned by PCR，the sequence feature was analyzed by BioEdit and MEGA software，and the expression in early development and adult tissues was detected by real-time quantitative PCR. The obtained emx1 cDNA was 1 127 bp，including a 708 bp open reading frame(ORF)，encoding a polypeptide of 235 amino acids. The alignment analysis of amino acid sequence discovered that the emx1 from P. olivaceus showed the highest 95% homology with that of Stegastes partitus and Poecilia formosa，the sequence similarities between P. olivaceus and Danio rerio, Astyanax mexicanus, Xenopus tropicalis, Gallus gallus, Mus musculus and Homo sapiens，were 87%，84%，83%，80%，72% and 72%，respectively. The Emx1 protein was highly homologous with that in other fish through phylogenic analysis. Real-time quantitative PCR revealed that the emx1 mRNA was expressed in all detected tissues of P. olivaceus. The expression of emx1 was the highest in the ovary and secondly highest in the testis，while relatively low in other tissues. In early development stage，i.e.，from gastrula to 10 d after hatching，a relatively high expression of the emx1 mRNA was also observed，and the highest expression occurred in the blastopore stage and heart-beating stage. The findings indicated that the emx1 played an important role in early development and gonad development stages of P. olivaceus.

Key words: emx1, Paralichthys olivaceus, cDNA cloning, gene expression

SUN Wen-hui, ZHANG Jun-ling, SHI Zhi-yi, SUN Jin-jin, XIANG Yu-ting, CHEN Xiao-wu. Identification and Expression Analysis of emx1 from Paralichthys olivaceus[J]. Biotechnology Bulletin, 2016, 32(6): 161-167.

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