The Development and Application of Multiplex PCR Panels of Microsatellites in Hyriopsis cumingii

doi:10.13560/j.cnki.biotech.bull.1985.2016.01.026

Abstract

Abstract: In order to increase the analysis efficiency of microsatellite in Hyriopsis cumingii, 3 groups of 4 multiplex PCRs panels were established from the developed microsatellite labels. The stable multiplex PCR panels were used to analyze the genetic diversities of 56 purple breeding lines of H. cumingii. The results showed that the average number of alleles(A)was 18.75, the effective number of alleles(An)was 9.010, the average of polymorphism information content(PIC)was 0.857, the average observed heterozygosity(Ho)and expected heterozygosity(He)were 0.809 and 0.876, respectively, and Shannon information index(I)was 2.422. The Cervus v3.0 software was used to have parentage assignment for 114 individuals in 3 full-sib families, and the results revealed that accuracy of parentage assignment by this 3 groups of multiplex PCR panels was 100%. Therefore, this multiplex panels can be applied in genetic diversity studies of H. cumingii population, parentage assignment and family management with increased detection efficiency and reduced test cost.

Key words: Hyriopsis cumingii, microsatellites, multiplex PCR, genetic diversity, parentage assignment

YIN Hao, BAI Zhi-yi, HAN Xue-kai, LI Jia-le. The Development and Application of Multiplex PCR Panels of Microsatellites in Hyriopsis cumingii[J]. Biotechnology Bulletin, 2016, 32(1): 156-162.

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