Cloning and Characterization of ZmPht3；1，a Phosphate Transporter Gene in Maize

Abstract

Abstract: Pht3（phosphate transporter 3）family belonging to low-affinity phosphate transporters play an important role in the regulation of phosphorus homeostasis in plants. In order to explore the characterization of gene structure and response mechanism of ZmPht3；1 to phosphorus（P）starvation，the gene ZmPht3；1 was isolated from low-phosphorus（low-P）tolerance maize inbred line Mo17 by homology cloning. Then the quantitative real-time PCR and subcellular localization of ZmPht3；1 were performed for further research in this study. The results showed that the complete CDS of ZmPht3；1 was 1 101 bp encoding 366 putative amino acids in length，and contained 6 hydrophobic transmembrane domains and had a typical conservation structure domain of mitochondrial carrier family. The PCR results exhibited that the gene expressed in roots and leaves，and the expression patterns were significantly different in the two extreme materials，respectively. While gene expression pattern in low-P tolerance maize inbred line Mo17 both roots and leaves revealed general and special response to P deficiency stress in early and late stages，respectively. Subcellular localization in the transformed tobacco（Nicotiana benthamiana）showed that ZmPht3；1 mainly expressed in cytoplasm membrane and it was a phosphate transporter in both low- and high-P environment，and played a key role in the process of adapting to P starvation in maize.

Key words: maize, phosphate transporters, gene expression, subcellular localization

CLC Number:

10.13560/j.cnki.biotech.bull.1985.2016.12.012

WU Shan,LIN Dan. Cloning and Characterization of ZmPht3；1，a Phosphate Transporter Gene in Maize[J]. Biotechnology Bulletin, 2016, 32(12): 65-71.

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