Functional Complement Between General Nitrogen Regulator GlnG in Escherichia coli and General Nitrogen Regulator NtrC in Pseudomonas

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.025

Abstract

Abstract: This study aims to investigate whether or not there is functional complement in general nitrogen-regulation network between nitrogen metabolism regulator GlnG from Escherichia coli DH10B and NtrC from Pseudomonas stutzeri A1501. Using gene glnG of DH10B to complement gene ntrC of A1501 or vice versa，the physiological and biochemical phenotypes of the obtained functionally complementary strains were measured. The results showed that while nitrate or urea as the sole nitrogen source，DH10B’s glnG complemented the nitrogen-utilizing ability and partially restored the nitrogenase activity（about 45% of wild type）of mutant A1501’s ntrC. Comparing with DH10B’s glnG，A1501’s ntrC only complemented mutant DH10B glnG while arginine as sole nitrogen source. Above results revealed that not only was there close phylogenetical relationship，but also the functional complement in the tested nitrogen metabolism between DH10B’s GlnG and A1501’s NtrC.

Key words: Pseudomonas stutzeri A1501 NtrC, Escherichia coli DH10B GlnG, general nitrogen metabolism, functional complement

KE Qi， ,CHEN Qing-hua, ZHAN Yu-hua, LU Wei, YAN Yong-liang. Functional Complement Between General Nitrogen Regulator GlnG in Escherichia coli and General Nitrogen Regulator NtrC in Pseudomonas[J]. Biotechnology Bulletin, 2016, 32(4): 190-197.

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