The Construction of the Gene Transfer System of Strain Streptomyces sp. 211726 Producing Azalomycin F

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.026

Abstract

Abstract:

The objective of this study is to establish the gene transfer system of strain Streptomyces sp. 211726 producing azalomycin F，which can be used for genetic manipulations such as gene knock-out and expression of foreign genes. Intergeneric genetic transfer system of Streptomyces sp. 211726 producing azalomycin F was constructed by conjugating integrative plasmid pSET152 with pIB139. Results showed that 25 mg/mL apramycin may be used to efficiently screen conjugants. PCR verification revealed that exogenous plasmid was successfully integrated in the chromosomal DNA of Streptomyces sp. 211726. The continuous passage culture experiment demonstrated that transformed pSET152 and pIB139 of conjugants were stably inherited.

Key words: azalomycin F, Streptomyces sp. 211726, conjugation, genetic stability

MA Yan-ling, LIU Fu-lai, ZHANG Min, SUN Yu-hui, HONG Kui. The Construction of the Gene Transfer System of Strain Streptomyces sp. 211726 Producing Azalomycin F[J]. Biotechnology Bulletin, 2016, 32(4): 198-202.

References

［1］ Wakesman SA, Heriei AT. The nomen culture and classification of the Actinoymcetes［J］. J Bacteriol, 1943, 46：337-341.
［2］ Kieser HM, Kieser T, Hopwood DA. A combined genetic and physi-cal map of the Streptomyces coelicolor A3（2）chromosome［J］. J Bacteriol, 1992, 174：5496-5507.
［3］ Goodfellow M, Cross T. The Biology of the Actinomyces［M］. London：Academic Press, 1984.
［4］ Franco CM, Coutinho LE. Detection of novel secondary metabolites［J］. Crit Rev Biotechnol, 1991, 11：193-276.
［5］ Davies J. What are antibiotics? Archaic functions for modern activities［J］. Mol Microbiol, 1990, 4：1227-1232.
［6］ Morin RB, Gorman M. Chemistry and biology of beta-lactam antibiotics［M］. New York：Academic Press, 1982.
［7］ Strynadka NC, Adachi H, Jensen SE, et al. Molecular structure of the acyl-enzyme intermediate in beta-lactam hydrolysis at 1. 7 A resolution［J］. Nature, 1992, 359：700-705.
［8］Arai M. Azalomycins B and F, two new antibiotics. I. Production and isolation［J］. The Journal of Antibiotics, 1960, 13：46.
［9］Krystofova S, Varecka L, Betina V. The uptake by trichoderma viride Mycelium. correlation with growth and conidiation［J］. General Physiology and Biophysics, 1995, 14：323-338.
［10］ Yuan G, Hong K, Lin H, et al. New azalomycin F analogs from ma-ngrove Streptomyces sp. 211726 with activity against microbes and cancer cells［J］. Marine Drugs, 2013, 11：817-829.
［11］ Yuan G, Lin H, Wang C, et al. 1H and 13C assignments of two new macrocyclic lactones isolated from Streptomyces sp. 211726 and revised assignments of azalomycins F3a, F4a and F5a［J］. Magnetic Resonance in Chemistry：MRC, 2011, 49：30-37.
［12］ Yuan GJ, Hong K, Lin HP, Li J. Azalomycin F4a 2-ethylpentyl ester, a new macrocyclic lactone, from mangrove actinomycete Streptomyces sp. 211726［J］. Chinese Chemical Letters, 2010, 21：947-950.
［13］Yuan GJ, Li PB, Yang J, et al. Anti-methicillin-resistant Staphylococcus aureus assay of azalomycin F5a and its derivatives［J］. Chinese Journal of Natural Medicines, 2014, 12：309-313.
［14］Fukushima K, Arai T, Iwasaki S, et al. Studies on macrocyclic lactone antibiotics. VI. Skeletal structure of copiamycin［J］. J Antibiot（Tokyo）, 1982, 35：1480-1494.
［15］袁干军. 产大环内酯化合物的红树林放线菌的筛选、产物分离鉴定及活性研究［D］. 海口：海南大学, 2010：1-185.
［16］Tobias K, Bibb MJ, Mark JB, et al. Practical Streptomyces Genetics［M］. Norwich：The John Innes Foundation, 2000.
［17］Mens T, Demeyer S, Du Bois B, et al. Refactoring：Current research and future trends［J］. Electronic Notes in Theoretical Computer Science, 2003, 82（3）：483-499.
［18］Wilkinson CJ, Hughes-Thomas ZA, Martin CJ, et al. Increasing the efficiency of heterologous promoters in actinomycetes［J］. J Mol Microbiol Biotechnol, 2002, 4（4）：417-426.
［19］Del Vecchio F, Petkovic H, Kendrew SG, et al. Active-site residue, domain and module swaps in modular polyketide synthases［J］. J Ind Microbiol Biotechnol, 2003, 30（8）：489-494.
［20］Gerdes K, Rasmussen PB, Molin S. Unique type of plasmid maintenance function：postsegregational killing of plasmid-free cells［J］. Proceedings of the National Academy of Sciences, 1986, 83（10）：3116-3120.
［21］ Sun Y, He X, Liang J. Analysis of function in plasmid pHZ1358 influencing its genetic and structural stability in Streptomyces lividans 1326［J］. Applied Microbiology and Biotechnoogy, 2009, 82：303-310.
［22］Sun Y, Zhou X, Liu J, et al. Streptomyces nanchangensis, a producer of the insecticidal polyether antibiotic nanchangmycin and the antiparasitic macrolide meilingmycin, contains multiple polyketide gene clusters［J］. Microbiology, 2002, 148：361-371.

[1]	LI Sheng-yan, LI Xiang-yin, LI Peng-cheng, ZHANG Ming-jun, ZHANG Jie, LANG Zhi-hong. Identification of Target Traits and Genetic Stability of Transgenic Maize 2HVB5 [J]. Biotechnology Bulletin, 2023, 39(1): 21-30.
[2]	LIU Yi-yun, DENG Li-min, YUE Hui-ying, YUE Chao, LIU Jian-hua. Research Progress in Plasmid Conjugation and Its Inhibitors [J]. Biotechnology Bulletin, 2022, 38(9): 35-46.
[3]	TIAN Wen-jia, DOU Gui-ming, WANG Sha, SUN Jing-ya, MA Yu-chao. Establishment of a CRISPR/Cas9-mediated Gene Cluster-knockout System in the Endophytic Streptomyces SAT1 [J]. Biotechnology Bulletin, 2019, 35(6): 1-8.
[4]	YU Wei-wei, DU Bang-hao, ZHANG Min-ne, WAN Qiao-ling, YANG Shuo, ZHAO Chen-ju. A Review on Enzymatic Degradation and Transformation Mechanisms of Free and Conjugated Estrogens in the Environment [J]. Biotechnology Bulletin, 2019, 35(4): 151-162.
[5]	LIANG Hai-sheng, LI Meng-tao, LI Sheng-yan, WANG Hai, ZHANG Jie, LANG Zhi-hong. Agronomic Traits Analysis of Transgenic Bt cry1Ah Maize HGK60 Line [J]. Biotechnology Bulletin, 2018, 34(7): 92-100.
[6]	Zhu Tao, Deng Ligong, Duan Lei, Chang Yunsong, Shao Zhongqi, Mao Huihua, Yu Xuefeng. Expression and Purification of the Diphtheria Toxin Variant CRM197 in Corynebacterium diphtheriae [J]. Biotechnology Bulletin, 2014, 0(3): 182-186.