Evaluation on Growth and Biochemical Properties of Eight Strains of Marine Microalgae Nannochloris sp.

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.034

Abstract

Abstract: This work is to evaluate the productive potential of Nannochloris sp. by analyzing the growth and biochemical characteristics of 8 strains screened from the South China Sea region. Using dry weight determination，soxhlet extraction，kjeldahl nitrogen method，phenol sulfuric acid determination combined with gas chromatography-mass spectrometry technology，the microalgal growth，the content of lipid，protein and polysaccharide and the composition of fatty acids were analyzed correspondingly. The maximum dry weight of eight strains was 0.39 - 0.91 g/L and the SCS-761 strain grew the fastest. The total lipid content ranged in 19.06% - 33.67%，and that of SCS-249，SCS-589，and SCS-655 was over 25%，and the lipid productivity of SCS-655 strain was as high as 22.23 ± 0.71 mg/(L·d). The protein contents of SCS-249，SCS-325，SCS-355，SCS-640，and SCS-769 were over 30%，of which the highest for SCS-640 strain(36.70 ± 2.20)%. Moreover，the polysaccharide content of each strain was between 19.23% to 29.34%，of which the highest was SCS-761 strain and the least was SCS-655 strain. In addition，the analysis of fatty acid composition showed that the structures of fatty acid in all microalgae strains were similar，mainly composed of C16 saturated fatty acids and C18 series of fatty acids. Linoleic acid was the highest content among unsaturated fatty acids，and the linoleic acid contents of SCS-249，SCS-355，and SCS-761 were over 40%. In conclusion，the results indicates that marine microalgae Nannochloris sp. has relatively abundant germplasm resources including lipid，protein and linoleic acid，and owns considerable application potential；thus it is necessary to have more in-depth research.

Key words: marine microalgae, growth, lipid, protein, polysaccharide, fatty acid

CHEN Cheng-hao, WU Jia-yi, TANG Ming-xing, LI Tao, WU Hua-lian, WANG Guang-hua, DAI Shi-kun, XIANG Wen-zhou. Evaluation on Growth and Biochemical Properties of Eight Strains of Marine Microalgae Nannochloris sp.[J]. Biotechnology Bulletin, 2016, 32(6): 231-237.

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