Optimization of the Purification Method for Transmembrane Protein AgrCTM6-7C

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.009

Abstract

Abstract: The core objective of this paper is to obtain the protein of AgrC_TM6-7Cwith high purity by optimizing the purification processes,and provide the materials for further research. We investigated the influence of imidazole concentration in buffer used in the metal ion affinity chromatography and buffer with different pH used in the ion exchange chromatography on the purification of AgrC_TM6-7C. Moreover,we discussed the effects of β-Mercaptoethanol on protein aggregation and kinase activity of AgrC_TM6-7C. The optimized purification method resulted in the obvious increase on the yield and purity of AgrC_TM6-7C,producing 15 mg with about 98% purity from one-liter culture medium.

Key words: Staphylococcus aureus, AgrCTM6-7C, purification

WANG Lu-lu, QUAN Chun-shan, XU Yong-bin, WANG Guan-tian, LIU Shuang, CHEN Ying. Optimization of the Purification Method for Transmembrane Protein AgrC_TM6-7C[J]. Biotechnology Bulletin, 2016, 32(7): 59-65.

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Optimization of the Purification Method for Transmembrane Protein AgrC_TM6-7C

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