Molecular Cloning and Expression Analysis of Aquaporins SIP2-1 Gene from Banana

doi:10.13560/j.cnki.biotech.bull.1985.2016.07.012

Abstract

Abstract: In the present study,we isolated an aquaporin gene from banana,and designated as MaSIP2-1. MaSIP2-1’s complete ORF was 717 bp,and it encoded 239 amino acids. Alignment of amino acid sequences and phylogenetic analysis indicated that the protein encoded by MaSIP2-1 was in high similarity with AQP-encoding protein in the other known plants;and highly homologous with amino acid sequences in Musa acuminate,Elaeis guineensis,Jatropha curcas,and Camellia sinensis by 98%,74%,65%,and 63%,respectively. Organ-specific analysis showed that MaSIP2-1 constitutively expressed in roots,stems,leaves,flowers and fruits,and the highest in stems. Stress analysis demonstrated that MaSIP2-1 responded to the stress such as the drought,salt and waterlogging.

Key words: aquaporins, SIP, banana, bioinformatics, stress, real-time PCR

XU Yi, HOU Xiao-wan, XU Bi-yu, JIN Zhi-qiang, HU Wei, SONG Shun. Molecular Cloning and Expression Analysis of Aquaporins SIP2-1 Gene from Banana[J]. Biotechnology Bulletin, 2016, 32(7): 81-86.

References

[1] Aharon R, Shahak Y, Wininger S, et al. Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress[J]. Plant Cell, 2003, 15：439-447.
[2] Bray EA. Molecular responses to water deficit[J]. Plant Physiol, 1993, 103：1035-1040.
[3] Cheeseman JM. Mechanisms of salinity tolerance in plants[J]. Plant Physiol, 1988, 8：547-550.
[4] Blumwald E. Sodium transport and salt tolerance in plants[J]. Curr Opin Cell Biol, 2000, 12：431-434.
[5] Uehlein N, Lovisolo C, Siefritz F. Kaldenhoff：the tobacco aquaporin NtAQP1 is a membrane CO 2 pore with physiological functions[J]. Nature, 2003, 425：734-737.
[6] Kaldenhoff R, Fischer M. Aquaporins in plants[J]. Acta Physiol, 2006, 187：169-176.
[7] Sade N, et al. The role of tobacco Aquaporin1 in improving water use efficiency, hydraulic conductivity, and yield production under salt stress[J]. Plant Physiol, 2010, 152：245-254.
[8] Eisenbarth DA, Weig AR. Dynamics of aquaporins and water relations during hypocotyl elongation in Ricinus communis L. seedlings[J]. J Exp Bot, 2005, 56：1831-1842.
[9] Gao Z, He X, Zhao B, et al. Overexpressing a putative aquaporin gene from wheat, TaNIP, enhances salt tolerance in transgenic Arabidopsis[J]. Plant Cell Physiol, 2010, 51：767-775.
[10] Johanson U, Karlsson M, Johansson I, et al. The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants[J]. Plant Physiol, 2001, 126：1358-1369.
[11] Sakurai J, Ishikawa F, Yamaguchi T, et al. Identification of 33 rice aquaporin genes and analysis of their expression and function[J]. Plant Cell Physiol, 2005, 46：1568-1577.
[12] Chaumont F, Barrieu F, Wojcik E, et al. Aquaporins constitute a large and highly divergent protein family in maize[J]. Plant Physiol, 2001, 12：1206-1215.
[13] Maurel C, Verdoucq L, Luu DT, et al. Plant aquaporins：membrane channels with multiple integrated functions[J]. Annu Rev Plant Biol, 2008, 59：595-624.
[14] Liu CW, Fukumoto T, Matsumoto T, et al. Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination[J]. Plat Physiology and Biochemistry, 2013, 63：151-158.
[15] Yang S, Cui L. The action of aquaporins in cell elongation, salt stress and photosynthesis[J]. Sheng Wu Gong Cheng Xue Bao, 2009, 25（3）：321-327.
[16] North GB, Nobel PS. Heterogeneity in water availability alters cellular development and hydraulic conductivity along roots of a desert succulent[J]. Ann Bot, 2000, 85：247-255.
[17] Horie T, Kaneko T, Sugimoto G, et al. Mechanisms of water transport mediated by PIP aquaporins and their regulation via phosphorylation events under salinity stress in barley roots[J]. Plant Cell Physiol, 2011, 52：663-675.
[18] Chaumont F, Moshelion M, Daniels MJ. Regulation of plant aquaporin activity[J]. Biol Cell, 2005, 97：749-764.
[19] Bienert GP, et al. Membrane transport of hydrogen peroxide[J]. Biochim Biophys Acta, 2006, 1758：994-1003.
[20] Lian HL, Yu X, Ye Q, et al. The role of aquaporin RWC3 in drought avoidance in rice[J]. Plant Cell Physiol, 2004, 45：481-489.
[21] Guo L, Wang ZY, Lin H, et al. Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family[J]. Cell Res, 2006, 16：277-286.
[22] Zhou S, Hu W, Deng X, et al. Overexpression of the wheat aquaporin gene, TaAQP7, enhances drought tolerance in transgenic tobacco[J]. PLoS One, 2012, 7：e52439.
[23] Xu Y, Hu W, Liu JH, et al. A banana aquaporin gene, MaPIP1;1, is involved in tolerance to drought and salt stresses[J]. BMC Plant Biology, 2014, 14：59.
[24] Hu W, Yuan Q, Wang Y, et al. Overexpression of a wheat aquaporin gene, TaAQP8, enhances salt stress tolerance in transgenic tobacco[J]. Plant Cell Physiol, 2012, 53：2127-2141.
[25] Jang HY, Yang SW, Carlson JE, et al. Two aquaporins of Jatropha are regulated differentially during drought stress and subsequent recovery[J]. J Plant Physiol, 2013, 170（11）：1028-1038.
[26] Xu CH, et al. Heterologous expression of the wheat aquaporin gene TaTIP2;2 compromises the abiotic stress tolerance of Arabidopsis thaliana[J]. PLoS One, 2013, 8（11）：e79618.
[27] Sreedharan S, Shekhawat UK, Ganapathi TR. Transgenic banana plants overexpressing a native plasma membrane aquaporin MusaPIP1;2 display high tolerance levels to different abiotic stresses[J]. Plant Biotechnology Journal, 2013, 11：942-952.
[28] 候晓婉, 胡伟, 颜彦, 等. 香蕉MaPIP2-6基因的克隆、亚细胞定位及表达分析[J]. 西北植物学报, 2015, 35（3）：427-433.
[29] Jang JY, Kim DG, Kim YO, et al. An expression analysis of a gene family encoding plasma membrane aquaporins in response to abiotic stresses in Arabidopsis thaliana[J]. Plant Molecular Biology, 2004, 54（5）：713-725.